Special topic: Metagenomics - Genome Sciences
Special topic: Metagenomics - Genome Sciences
Special topic: Metagenomics - Genome Sciences
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41 samples (from surface waters): Filter<br />
(0.1-3µM), E. coli is 2µm by 0.1µm.<br />
6.25 Gigabases: 2.7M pages (at 2200<br />
characters per page): 270m stack (at<br />
0.1mm per page).<br />
How much does species composition at a<br />
single site over time?<br />
To learn about the:<br />
–Diversity,<br />
–Geographic distribution, and<br />
–Protein content & metabolism<br />
Sorcerer II: The beauty of shot-gun sequencing<br />
Global Ocean Sampling (GOS) survey:<br />
‣ the species diversity,<br />
‣ geographic distribution,<br />
‣ gene content and metabolism<br />
From Nova Scotia to Hawaii:<br />
6.25 x 10^9 bp from 41 sites.<br />
Protocol:<br />
‣ Collect sea surface water samples,<br />
‣ filter (0.1-3.0µm) to recover<br />
bacteria,<br />
‣ Extract DNA, fragment and clone<br />
into a plasmid library.<br />
‣ Sequence DNA in plasmid library.
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