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File : THESIS.pdf - Universitas Muhammadiyah Malang

File : THESIS.pdf - Universitas Muhammadiyah Malang

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Drawing<br />

conclusions<br />

Suggesting<br />

improvements<br />

3 marks<br />

3 marks<br />

Manipulation, measurement and observation<br />

Successful collection of data and<br />

observations Candidates should be able<br />

to:<br />

• set up apparatus correctly;<br />

• follow instructions given in the form of written instructions or diagrams;<br />

• use their apparatus to collect an appropriate quantity of data or<br />

observations, including subtle differences in colour or other properties of<br />

materials;<br />

• make measurements using millimetre scales, graticules, protractors,<br />

stopwatches, balances, measuring cylinders, syringes, thermometers,<br />

and other common laboratory apparatus.<br />

Candidates will be expected to use light microscopes. They should be<br />

able to place the slide on the stage, arrange the lighting appropriately and<br />

focus on the specimen at both low- power (X10, sometimes described as 16<br />

mm or 2/3”) and high-power (X40, or 4 mm or 1/6”) using a microscope with a<br />

graticule fitted into the eyepiece.<br />

Decisions relating to measurements or observations<br />

Candidates should be able to:<br />

• decide how many tests, measurements or observations to perform;<br />

• make measurements or observations that span the largest possible<br />

range within the limits either of the equipment provided or of the<br />

instructions given;<br />

• make quantitative measurements or qualitative observations that are<br />

appropriately distributed within this range;<br />

• decide how long to leave experiments running before making readings.<br />

• replicate readings or observations as necessary;<br />

• make and record sufficient, accurate measurements and observations.<br />

Candidates may need to choose how many tests, measurements and<br />

observations can be made in the time available. In some experiments a<br />

regularly-spaced set of measurements will be appropriate. For other<br />

experiments, such as those requiring the peak value of a curved graph<br />

to be determined, it may be appropriate for the measurements to be<br />

concentrated in one part of the range investigated. Candidates will be<br />

expected to be able to identify the most appropriate distribution of values.<br />

In qualitative experiments, precise descriptions and comparisons of colour or<br />

other observations are expected.<br />

In experiments, such as those involving enzymes:<br />

initial rate of reaction may be measured (in which case<br />

measurements should be conducted as quickly as practicable),<br />

the rate of reaction might be expected to be constant over several<br />

minutes, or colour changes may take several minutes to occur, in<br />

which case leaving the experiment to run for as long as possible<br />

may be appropriate,<br />

an end point is being sought, in which case, candidates should<br />

expect to run the experiment until the end point is achieved or the<br />

time runs out.<br />

Repeated readings of particular quantities are often necessary in<br />

biology, where experimental errors and variation in the activity of<br />

biological materials are large and an average value would be more<br />

representative. Individual readings or observations should be repeated where<br />

they appear to be anomalous. It may be necessary for the candidate to<br />

decide how many times to let something that is repetitious occur before<br />

124

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