File : THESIS.pdf - Universitas Muhammadiyah Malang

(g) use the knowledge gained in this section in new situations or to solve related problems.
Applications of Biology
BIOLOGY 9700 A/AS LEVEL
Q BIODIVERSITY AND CONSERVATION
Content
Classification
Conservation
issues Learning
Outcomes Candidates
should be able to:
(a) *outline the five kingdom classification to illustrate the diversity of
organisms (cross reference Syllabus Section A (c) and A (g));
(b) discuss the meaning of the term biodiversity;
(c) discuss the reasons for the need to maintain biodiversity;
(d) describe the reasons why one named species has become endangered,
and use this information in the context of other endangered species;
(e) discuss methods of protecting endangered species including the
roles of zoos, botanic gardens, conserved areas (national parks) and
seed banks;
(f) use the knowledge gained in this section in new situations or to
solve related problems.
R GENE TECHNOLOGY (includes some material originally in Section O)
Content
Gene technology for insulin production
Markers for genetic engineering
Benefits and hazards of gene
technology DNA sequencing and
genetic fingerprinting Cystic Fibrosis
Genetic screening and genetic counselling
Learning Outcomes
Candidates should be able to:
(a) describe the steps involved in the production of bacteria capable of
synthesising human insulin:
• identifying the human insulin gene
• isolating mRNA and making cDNA using reverse transcriptase
• cloning the DNA using DNA polymerase
• inserting the DNA into a plasmid vector using restriction enzymes
and DNA
ligase
• inserting the plasmid vector into the host bacterium
• identifying genetically modified bacteria using antibiotic resistance
genes
• cloning the bacteria and harvesting the human insulin
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