Programfüzet (PDF) - DE OEC Tudományos Diákköri Tanács

O1.5. Klárik Zoltán Gen.Med. V.Sebészeti Intézet, Sebészeti Mtéttani TanszékEXPERIMENTAL MODEL FOR HEMODYNAMICAL ANDMICROCIRCULATORY INVESTIGATIONS IN CASES OF ARTEFICIALARTERIO-VENOUS FISTULAS IN RATSINTRODUCTION: Permanent access for hemodialysis requires a creation of ahigh flow artificial anastomosis between an artery and a vein. The preferredtype of arterio-venous fistula (AVF) is the Cimino fistula (CF) but there aremany other possibilities. As a response to higher pressure of arterial blood flow,the vein will dilate and its wall will thicken. However, these change onlyapparent after 4 to 8 weeks according to the human literature.MATERIALS AND METHODS: In this experimental model, several AVFwere created in the left lower extremity of 18 anaesthetized CD outbred malerats (bodyweight: 362.4 ± 61.3 g) to demonstrate the changes in arterial andvenous blood flow. On the 8th postoperative week the following examinationswere performed: both on control and shunted side a Transonic T206 flowmeterwas used for determining blood flow rate in larger vessels (femoral artery andsaphenous vein), furthermore, to measure the local microcirculatory bloodperfusion including the perfusion in capillaries (nutritive flow), arterioles,venules and shunting vessels a Perimed Periscan PIM II type laser Doppler scanwas applied.RESULTS: There was not visible difference between the intact and operatedlimb motion during regular observation of animals. Paws were the same pinkcolour on both sides. Arterial to venous flow rate (ml/min) difference was 2.27in intact side and 1.13 in the shunted side. Microcirculation of the extremities’skin showed better results in case of well permeable shunts.CONCLUSION: The presented experimental model seems to be suitable toexamine the blood flow changes for further laboratory and histologicalcomparative examinations.Témavezet: Dr. Hevér Tímea, Dr. Németh Norbert124