Programfüzet (PDF) - DE OEC Tudományos Diákköri Tanács

MB1.6. Keret, Ophir Gen.Med. IV.Biokémiai és Molekuláris Biológiai IntézetA GENE EXPRESSION BASED COMPARISION OF WILD TYPE ANDPRMT1 KO EMBRYONIC STEM CELLSMouse embryonic stem cells (ES) are in vitro cultured lineage derivatives ofmouse blastocysts. The ES genomes embody's the potential to differentiate intoall three lineages of a developing embryo. The differentiation is in essence acontinuing process of gene expression followed by phenotypic change; thus thedevelopment may be blocked by knocking out genes relevant to these processes.Epigenetic changes, such as histone post-translational modifications can befound in the core of embryonic gene expression and differentiation. Previously,we investigated a block in differentiation (specifically neurons) by studying agene responsible for methylation of an arginine residue on a histone H4 tail(known as protein methyl arginine tranferase 1 or PRMT1). The gene wasmutated via insertional mutagenesis in mouse ES cell line, which was unable totransform phenotypically to neurons. Upon re-introduction of a valid wild typecopy of the gene the neuronal differentiation was allowed. Here, we comparedthe PRMT1 KO type ES cells to a wild type D3 using QPCR methods. Weanalyzed a set of differentiation markers (activated genes in our case) known fortheir correlation with either neuronal differentiation or a plural potential stemcell not yet differentiated. Genes such as: Oct 3/4, Nanog, Cdx2, Sox17, Nestin,Brachyury and others. We hope that carefull characterization of the in vitrodifferentiation processes will yield the possibility of in-vitro creation of either:neuronal components of stromal tissue or specific neuronal parenchyma withvarious capabilities. This knowledge may be called for in the future ofregenerative medicine.Témavezet: Dr. Bálint L. Bálint120