Természetes hatóanyagok jelentőségének vizsgálata máj- és ...

Summary
Ph.D Thesis
Importance of herbal preparations in liver and bowel diseases
Prepared by Erika Rapavi
Supervised by Dr. Anna Blázovics Ph.D
Biochemical Research Group, II. Department of Medicine, Semmelweis University,
2006
The redox homeostasis of cells and tissues is vital for fundamental life processes, its
disturbance may cause damage to organism. The goal of our investigations was to
examine the effect of Hungarian and foreign herbal teas on blast transformation of
human peripheral blood lymphocytes in vitro, and to study their impact on redoxi
processes of the healthy mammal organism in vivo. Our further aim was to evaluate the
effect of the therapeutic dosage of a diosmin-hesperidin-containing drug (Detralex®) on
redox parameters of alimentary-induced fatty liver in rats. Some authors reported that
dietary phenolics exhibit pro-oxidant and cytotoxic properties under certain conditions.
Based on these observations we examined the effect of high dosages of this drug on the
redox state of liver in healthy animals, as well as on redoxi processes of liver
regeneration in rats with experimental hepatic injury induced by lipid-rich diet and
thioacetamide. Biochemical, analytical, and histological methods were used.
The results show that the aqueous extracts of the herbal teas examined have
antioxidant properties as a function of concentration and steeping time in non-enzymatic
systems in vitro. The effect of the infusion of calyx of Hibiscus sabdariffa L. and
Beiqishen herbal tea on the cell-mediated immune response suggests that these
infusions have in vitro immunomodulating, cytostatic properties. Both infusions
decreased mitogen-induced blastogenesis in normal subjects. These extracts did not
affect, however, the level of spontaneous proliferation of human lymphocytes. Aqueous
extracts of the herbal teas studied significantly influenced redox homeostasis of the liver
and plasma in healthy rats. Treatment with a drug containing diosmin (450 mg) and
hesperidin (50 mg), in therapeutic dosage, may improve the antioxidant defensive
system and the redox state in alimentary-induced fatty liver disease. High dosages of the
drug (425 mg/body weight/day and 315 mg/body weight/day, respectively) deteriorated
or did not change the redox state of liver during the recovery period.
In conclusion, it may be stated that high dosage of herbal drugs and preparations
may have pro-oxidant effect in both healthy and sick organisms.
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