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BD Diagnostics EN

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244520 500 g Difco LB Agar (Miller)<br />

244510 2 kg LB (Luria-Bertani) Agar, Miller is used for maintaining and<br />

propagating Escherichia coli in molecular microbiology procedures.<br />

LB Agar, Miller is based on LB Medium as described by Miller for<br />

the growth and maintenance of E. coli strains used in molecular<br />

microbiology procedures. These are nutritionally rich media<br />

designed for growth of pure cultures of recombinant strains. E.<br />

coli grows more rapidly because they provide the cells with amino<br />

acids, nucleotide precursors, vitamins and other metabolites that<br />

the microorganism would otherwise have to synthesise.<br />

240230 500 g Difco LB Broth (Lennox)<br />

240210 2 kg LB Broth, Lennox contains ten times the sodium chloride level<br />

of Luria Broth Base, Miller and one half of that found in LB<br />

Broth, Miller. This allows the researcher to select the optimal salt<br />

concentration for a specific strain. If desired, the medium may be<br />

aseptically supplemented with<br />

glucose to prepare the complete medium described by Lennox.<br />

244620 500 g Difco LB Broth (Miller)<br />

244610 2 kg LB (Luria-Bertani) Broth, Miller is used for maintaining and<br />

propagating Escherichia coli in molecular microbiology procedures.<br />

241420 500 g Difco Luria Broth Base (Miller)<br />

Luria Broth Base, Miller is used for maintaining and propagating<br />

Escherichia coli in molecular microbiology procedures with or<br />

without added glucose.<br />

248510 500 g Difco M9 Minimal Salts (5×)<br />

Used in preparing M9 Minimal Medium which is used for<br />

cultivating recombinant strains of Escherichia coli. M9 Minimal<br />

Salts, 5× is a 5× concentrate that is diluted to a 1× concentration<br />

and supplemented with an appropriate carbon and energy<br />

source, such as dextrose, to provide a minimal, chemically defined<br />

medium. The medium will support the growth of “wild-type”<br />

strains of E. coli. M9 Minimal Salts is useful for maintaining<br />

positive selection pressure on plasmids coding for the ability to<br />

produce essential substances such as amino acids or vitamins. M9<br />

Minimal Medium is also used to maintain stocks of F´-containing<br />

bacteria for use with M13. The medium can be supplemented with<br />

specific amino acids or other metabolites, allowing for selection of<br />

specific auxotrophs.<br />

240410 500 g Difco NZCYM Broth<br />

For cultivation of molecular genetic strains of E. coli for replication<br />

of recombinant bacteriophage.<br />

292438 500 g BBL Select APS - LB Broth Base<br />

<strong>BD</strong> Difco LB Broth Base (<strong>BD</strong> Select APS - Alternative Protein<br />

Source). For the propagation and maintenance of E. coli for<br />

molecular biology. Non-animal origin formulation, 500 g.<br />

212485 500 g BBL Select APS - Super Broth Base<br />

212486 10 kg <strong>BD</strong> Select APS Super Broth is a molecular genetic medium that<br />

will grow E. coli to a high cell density. There is no glucose in the<br />

formulation thus preventing acetate build-up in the fermentation<br />

of the organism.<br />

244310 500 g Difco SOB Medium<br />

For cultivation of molecular genetic strains of E. coli.<br />

243820 500 g Difco Terrific Broth<br />

243810 2 kg For cultivation of molecular genetic strains of E. coli. Formulation<br />

developed to increase plasmid yield.<br />

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