13.07.2015 Views

Marie-Christine LE PASLIER - Inra

Marie-Christine LE PASLIER - Inra

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De nouveaux formats surdes technologies existantesouDe nouvelles technologies= personal sequencing machine• Roche : 454 GS-Junior• 35 Mb/run• Illumina : MiSeq• 1,5 Gb/run• Ion Torrent : PGM (Personal Genome Machine)• incorporation d’un nucléotide par la polymérase->libération d’un proton ->variation de pH• semiconductor sequencing technology• 50 à 300 Mb/run• Rothberg et al, Nature,475, 21Juillet2011EPGV Unité 1279_GAP31

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