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VHB VHD Virologie D1 2011 Dr Evelyne Schvoerer

VHB VHD Virologie D1 2011 Dr Evelyne Schvoerer

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Diagnostic biologiqueAtteinte hépatiqueCytolyse : ↑ TA (ALAT>ASAT)- Diagnostic virologique directAntigènes• Sérum :– Ag HBs (enveloppe) : 1 er marqueur qui apparaît (1-3 mois)– Ag HBeTechniques immunoenzymatiques (ELISA)ADN viral : PCR ++, hybridation moléculaire• Diagnostic précoce, greffons, Nnés, virus mutants• Quantification : charge virale• Séquençage : recherche de mutations, génotypage- Diagnostic virologique indirect : Anticorps →11

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