Fonction et régulation de la protéine ICAP-1alpha dans la ...

tel-00435843, version 1 - 24 Nov 2009
Figure S4: β1-integrin activation in Icap-1 +/+ and Icap-1 rescue cells induces a spreading
phenotype similar to Icap-1 −/− cells.
Spreading assays of Icap-1 +/+ and Icap-1 −/− MEF cells (A) and Icap-1 +/+ , Icap-1 −/− , and Icap-
1 rescue osteoblasts (B and C) with or without treatment using either 0.5 mM MnCl2 (A and B)
or with 10 µg/ml 9EG7 mAb (C). Cells were treated in suspension after plating in a dose
dependent manner prior plating on FN or CL. Cell spreading was measured as described in
Fig. 2 C. Error bars indicate SD from three independent experiments.