Fonction et régulation de la protéine ICAP-1alpha dans la ...
Fonction et régulation de la protéine ICAP-1alpha dans la ...
Fonction et régulation de la protéine ICAP-1alpha dans la ...
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tel-00435843, version 1 - 24 Nov 2009<br />
Figure S2: Icap-1 +/+ and Icap-1 rescue MEF cells disp<strong>la</strong>y simi<strong>la</strong>r spreading and migration<br />
velocities.<br />
A. Spreading assay of Icap-1 +/+ , Icap-1 −/− , and Icap-1 rescue MEF cells on increasing FN<br />
substrate <strong>de</strong>nsities. Cells were see<strong>de</strong>d on various concentrations of FN-coated surfaces and<br />
were allowed to spread for 1 h. Round and f<strong>la</strong>ttened cells were counted, and spread cells were<br />
expressed as the percentage of total cell number. Each point represents the mean of two<br />
separate experiments, and error bars represent SD. *, P < 0.05.<br />
B. The migration speed of Icap-1 +/+ , Icap-1 −/− , and Icap-1 rescue MEF cells was d<strong>et</strong>ermined on<br />
various FN concentrations using time-<strong>la</strong>pse phase-contrast vi<strong>de</strong>o microscopy and cell tracking<br />
with M<strong>et</strong>aMorph software. 20–40 cells were photographed for each experimental condition at<br />
4-min intervals over an 8-h period. Error bars represent SD from three separate experiments.<br />
The maximal migration speed was used to s<strong>et</strong> up 100% for each cell type.
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