Fonction et régulation de la protéine ICAP-1alpha dans la ...

tel-00435843, version 1 - 24 Nov 2009
Figure S1: ICAP-1 loss induces no changes in the distribution of β3 integrin containing FA
and in the expression of adhesion proteins.
A. Confocal images of Icap-1 +/+ and Icap-1 -/- MEF cells. Cells were cultured overnight on
1µg/ml FN and processed for immunostaining to visualize β3 integrin and vinculin.
B. ICAP-1 and FA proteins expression in Icap-1 +/+ and Icap-1 -/- MEF cells. An equal amount
of protein from cell lysates in radioimmunoprecipitation assay was subjected to Western
blotting analysis using either anti-ICAP-1 pAb, anti-talin, anti-vinculin or anti-paxillin mAb.
The same membrane has been blotted with anti-actin mAb to control loading.
C. Cell surface analysis of β1- and β3- integrin expression on Icap-1 +/+ and Icap-1 -/- MEF cells
estimated by flow cytometry. (left) Icap-1 +/+ (black line and red line) or Icap-1 -/- cells (dashed
line and blue line) were stained with control antibody (black line) or with anti-β1 MB1.2
mAb. (right) Icap-1 +/+ (black and blue lines) or Icap-1 -/- (dashed and red lines) cells were
stained with control antibody (black line) or with anti-β3 rat mAb. Bar, 20µm.