Fonction et régulation de la protéine ICAP-1alpha dans la ...

tel-00435843, version 1 - 24 Nov 2009
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Published January 28, 2008
Figure 5. ICAP-1 loss induces de novo FA formation upon nocodazole treatment. (A) Icap-1 +/+ and Icap-1 � / � MEF cells were either untreated or treated
with 10 µ M nocodazole for 4 h or treated with 10 µ M nocodazole for 4 h followed by the drug washout for 60 and 90 min and were fi xed. FA and
microtubules were visualized by immunostaining of vinculin (green) and tyrosinated tubulin (red) and imaged using confocal microscopy. (B) Image quantifi
cation of vinculin-stained Icap-1 +/+ and Icap-1 � / � cells. Peripheral (left) or central (right) FA number per cell was counted (top) at each indicated step of
the nocodazole assay, and the corresponding mean surface (bottom) was quantifi ed. 20 cells were analyzed for each experimental condition, and error
bars indicate SD. Bar, 20 µ m.
JCB • VOLUME 180 • NUMBER 2 • 2008
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