KOMATI, H., MINASI, A., NARO, F., LAGARDE, M., PRIGENT, A. F., ADAMO, S., NEMOZ, G. Phorbol ester-induced differentiation of L6 myogenic cells involves phospholipase D activation. FEBS L<strong>et</strong>t., 2004, vol 577(3), p. 409-414. KOMATI, H., NARO, F., MEBAREK, S., DE ARCANGELIS, V., ADAMO, S., LAGARDE, M., PRIGENT, A. F., NEMOZ, G. Phospholipase D Is Involved in Myogenic Differentiation through Remo<strong>de</strong>ling of Actin Cytoskel<strong>et</strong>on. Mol Biol Cell., 2005, vol. 16(3), p. 1232-1244. KONDO, T., INUI, H., KONISHI, F., INAGAMI, T. Phospholipase D Mimics Platel<strong>et</strong>- Derived Growth Factor as a Comp<strong>et</strong>ence Factor in Vascular Smooth Muscle Cells. J Biol Chem., 1992, vol. 267(33), p. 23609-23616. KOTTER, K., JIN, S., KLEIN, J. Inhibition of Astroglial Cell Proliferation by Alcohols: Interference with the Protein Kinase C-Phospholipase D Signaling Pathway. Int J Dev Neurosci., 2000, vol. 18(8), p. 825-831. KOTTER, K., KLEIN, J. Ethanol Inhibits Astroglial Cell Proliferation by Disruption of Phospholipase D-Mediated Signaling. J Neurochem., 1999, vol. 73(6), p. 2517-2523. KOVBASNJUK, O., EDIDIN, M., DONOWITZ, M. Role of Lipid Rafts in Shiga Toxin 1 Interaction with the Apical Surface of Caco-2 Cells. J Cell Sci., 2001, vol. 114(Pt 22), p. 4025-4031. KREMER, J. M., LAWRENCE, D. A., JUBIZ, W., DIGIACOMO, R., RYNES, R., BARTHOLOMEW, L. E., SHERMAN, M. Di<strong>et</strong>ary Fish Oil and Olive Oil Supplementation in Patients with Rheumatoid Arthritis. Clinical and Immunologic Effects. Arthritis Rheum., 1990, vol. 33(6), p. 810-820. KTISTAKIS, N. T., BROWN, H. A., STERNWEIS, P. C., ROTH, M. G. Phospholipase D Is Present on Golgi-Enriched Membranes and Its Activation by Adp Ribosylation Factor Is Sensitive to Brefeldin A. Proc Natl Acad Sci U S A., 1995, vol. 92(11), p. 4952-4956. 184
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N° : 2006-ISAL-0031 ANNEE 2006 THE
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GOUTTE R. (Prof. émérite) CREATIS
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SIGLE ECOLE DOCTORALE NOM ET COORDO
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Dédicace Je dédie ce travail A me
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Je remercie également Mme Madelein
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AVANT-PROPOS Le travail présenté
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TABLE DES MATIERES REMERCIEMENTS...
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II-3-5-1 Huile d’olive...........
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IV-1-3-4 Distribution de la protéi
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Figure 26 : Gradient de saccharose
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Tableau XX : Effet des régimes sur
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MAP Kinase: Mitogen Activated Prote
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al., 1998 ; Steed et al., 1998). Le
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II- Rappels bibliographiques II-1-
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Figure 1 : Hématopoïèse du syst
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Tableau I : Antigènes couramment u
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Il existe cinq classes d’immunogl
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II-1-3-2-3 Les lymphocytes non T no
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Par la suite, de telles structures
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II-1-4-2 La composition des radeaux
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acylation (N-palmitoylation et S-my
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Tableau IV : Techniques d’analyse
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distingue d’une seconde zone de l
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essentiel du DAG est d’activer le
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R1 -O- R2 -O- Transphosphatidylatio
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II-2-4 Clonage et caractérisation
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Figure 8: Représentation schémati
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II-2-5-3 Les domaines PH et PX Il e
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Tableau VI: Caractéristiques des P
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Figure 10: La superfamille des prot
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Figure 11: la famille des PKC. En u
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phosphatidylinositol-4-monophosphat
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auteurs, PLD2 serait la forme préd
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prolifération, la croissance cellu
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II-3-2 Nomenclature des AG La nomen
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II-3-3 Les acides gras essentiels (
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II-3-4-3 Rôle fonctionnel II-3-4-3
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Tableau IX: Distribution (mole %) d
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II-3-5-1 Huile d’olive Le Maroc e
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L’analyse de la composition en AG
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1998; Kelley, 2001; Calder et al.,
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(1991) ont montré que les AGS ont
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uniquement chez les lymphocytes pro
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II-3-7-1 Effets des AG sur la synth
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II-3-7-2 Effets des AG sur les rade
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II-3-7-3 Effets des AG sur la struc
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Tableau XI : Influence des régimes
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III- MATERIELS ET METHODES III-1 Ma
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III-2 Méthodes III-2-1 Préparatio
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III-2-2 Préparation des échantill
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A B Figure 23: A : Métabolisme du
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III-2-5-4 Séparation du Pbut et du
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III-2-7 Analyse de la composition e
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croissantes (0.2M à 0.9M), puis l
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éalisée dans les mêmes condition
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vortexé avant d'être coulé. Apr
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Tableau XIII: Séquence des amorces
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IV- RESULTATS IV-1 Effet des acides
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Viabilité cellulaire (% du contrô
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métaboliquement stable et sa synth
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14 Activité PLD (pmoles de PC hydr
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L’ensemble des résultats concern
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Tableau XV b: Composition en acides
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PBut (%de la radioactivité des pho
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Prolifération des thymocytes (D.O5
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IV-1-3-2 Détection du PIP2 Le PIP2
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