JAMAL, Z., MARTIN, A., GOMEZ-MUNOZ, A., BRINDLEY, D. N. Plasma Membrane Fractions from Rat Liver Contain a Phosphatidate Phosphohydrolase Distinct from That in the Endoplasmic R<strong>et</strong>iculum and Cytosol. J Biol Chem., 1991, vol. 266(5), p. 2988-2996. JANEWAY, C., CHARLES, A., TRAVERS, P., WALPORT, M., SHLOMCHIK, M. Immunobiology 5 : the immune system in health and disease. New York and London: Garland Publishing. 2001, 5th ed. JEFFERY, N. M., CORTINA, M., NEWSHOLME, E. A., CALDER, P. C. Effects of Variations in the Proportions of Saturated, Monounsaturated and Polyunsaturated Fatty Acids in the Rat Di<strong>et</strong> on Spleen Lymphocyte Functions. Br J Nutr., 1997, vol. 77(5), p. 805- 823. JEFFERY, NM., SANDERSON, P., NEWSHOLME, EA., CALDER, PC. Characteristics of lipid and lymphocytes collected from the lymph of rats fed a low fat di<strong>et</strong> rich in n-6 or n-3 polyunsatured fatty acids. Nutr Res., 1998, vol. 18, p. 299-308. JEFFERY, N. M., SANDERSON, P., SHERRINGTON, E. J., NEWSHOLME, E. A., CALDER, P. C. The Ratio of N-6 to N-3 Polyunsaturated Fatty Acids in the Rat Di<strong>et</strong> Alters Serum Lipid Levels and Lymphocyte Functions. Lipids., 1996a, vol. 31(7), p. 737-745. JEFFERY, N. M., YAQOOB, P., NEWSHOLME, E. A., CALDER, P. C. The Effects of Olive Oil Upon Rat Serum Lipid Levels and Lymphocyte Functions Appear to Be Due to Oleic Acid. Ann Nutr M<strong>et</strong>ab., 1996b, vol. 40(2), p. 71-80. JENCO, J. M., RAWLINGSON, A., DANIELS, B., MORRIS, A. J. Regulation of Phospholipase D2: Selective Inhibition of Mammalian Phospholipase D Isoenzymes by Alpha- and B<strong>et</strong>a-Synucleins. Biochemistry., 1998, vol. 37(14), p. 4901-4909. JENKINS, G. H., FISETTE, P. L., ANDERSON, R. A. Type I Phosphatidylinositol 4- Phosphate 5-Kinase Isoforms Are Specifically Stimulated by Phosphatidic Acid. J Biol Chem., 1994, vol. 269(15), p. 11547-11554. 180
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N° : 2006-ISAL-0031 ANNEE 2006 THE
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GOUTTE R. (Prof. émérite) CREATIS
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SIGLE ECOLE DOCTORALE NOM ET COORDO
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Dédicace Je dédie ce travail A me
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Je remercie également Mme Madelein
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AVANT-PROPOS Le travail présenté
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TABLE DES MATIERES REMERCIEMENTS...
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II-3-5-1 Huile d’olive...........
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IV-1-3-4 Distribution de la protéi
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Figure 26 : Gradient de saccharose
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Tableau XX : Effet des régimes sur
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MAP Kinase: Mitogen Activated Prote
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al., 1998 ; Steed et al., 1998). Le
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II- Rappels bibliographiques II-1-
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Figure 1 : Hématopoïèse du syst
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Tableau I : Antigènes couramment u
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Il existe cinq classes d’immunogl
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II-1-3-2-3 Les lymphocytes non T no
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Par la suite, de telles structures
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II-1-4-2 La composition des radeaux
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acylation (N-palmitoylation et S-my
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Tableau IV : Techniques d’analyse
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distingue d’une seconde zone de l
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essentiel du DAG est d’activer le
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R1 -O- R2 -O- Transphosphatidylatio
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II-2-4 Clonage et caractérisation
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Figure 8: Représentation schémati
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II-2-5-3 Les domaines PH et PX Il e
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Tableau VI: Caractéristiques des P
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Figure 10: La superfamille des prot
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Figure 11: la famille des PKC. En u
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phosphatidylinositol-4-monophosphat
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auteurs, PLD2 serait la forme préd
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prolifération, la croissance cellu
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II-3-2 Nomenclature des AG La nomen
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II-3-3 Les acides gras essentiels (
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II-3-4-3 Rôle fonctionnel II-3-4-3
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Tableau IX: Distribution (mole %) d
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II-3-5-1 Huile d’olive Le Maroc e
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L’analyse de la composition en AG
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1998; Kelley, 2001; Calder et al.,
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(1991) ont montré que les AGS ont
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uniquement chez les lymphocytes pro
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II-3-7-1 Effets des AG sur la synth
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II-3-7-2 Effets des AG sur les rade
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II-3-7-3 Effets des AG sur la struc
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Tableau XI : Influence des régimes
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III- MATERIELS ET METHODES III-1 Ma
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III-2 Méthodes III-2-1 Préparatio
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III-2-2 Préparation des échantill
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A B Figure 23: A : Métabolisme du
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III-2-5-4 Séparation du Pbut et du
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III-2-7 Analyse de la composition e
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croissantes (0.2M à 0.9M), puis l
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éalisée dans les mêmes condition
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vortexé avant d'être coulé. Apr
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Tableau XIII: Séquence des amorces
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IV- RESULTATS IV-1 Effet des acides
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Viabilité cellulaire (% du contrô
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métaboliquement stable et sa synth
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14 Activité PLD (pmoles de PC hydr
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L’ensemble des résultats concern
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Tableau XV b: Composition en acides
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PBut (%de la radioactivité des pho
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