GENY, B., FENSOME, A., COCKCROFT, S. Rat Brain Cytosol Contains a Factor Which Reconstitutes Guanine-Nucleoti<strong>de</strong>-Binding-Protein-Regulated Phospholipase-D Activation in Hl60 Cells Previously Permeabilized with Streptolysin O. Eur J Biochem., 1993, vol. 215(2), p. 389-396. GIL, A. Polyunsaturated Fatty Acids and Inflammatory Diseases. Biomed Pharmacother., 2002, vol. 56(8), p. 388-96. GILBERT, J. J., PETTITT, T. R., SEATTER, S. D., REID, S. D., WAKELAM, M. J., HARNETT, M. M. Antagonistic Roles for Phospholipase D Activities in B Cell Signaling: While the Antigen Receptors Transduce Mitogenic Signals Via a Novel Phospholipase D Activity, Phosphatidylcholine-Phospholipase D Mediates Antiproliferative Signals. J Immunol., 1998, vol. 161(12), p. 6575-6584. GILSON, M. K., STRAATSMA, T. P., MCCAMMON, J. A., RIPOLL, D. R., FAERMAN, C. H., AXELSEN, P. H., SILMAN, I., SUSSMAN, J. L. Open "Back Door" in a Molecular Dynamics Simulation of Ac<strong>et</strong>ylcholinesterase. Science., 1994, vol. 263(5151), p. 1276-1280. GOLDSBY, R. A., KINDT, T.J, OSBORNE, B.A. Kuby immunology. New York, fourth edition New York. W H Freeman and Company, 2000. GOMEZ-MUNOZ, A., MARTIN, A., O'BRIEN, L., BRINDLEY, D. N. Cell-Permeable Cerami<strong>de</strong>s Inhibit the Stimulation of DNA Synthesis and Phospholipase D Activity by Phosphatidate and Lysophosphatidate in Rat Fibroblasts. J Biol Chem., 1994, vol. 269(12), p. 8937-8943. GORODINSKY, A., HARRIS, D. A. Glycolipid-Anchored Proteins in Neuroblastoma Cells Form D<strong>et</strong>ergent-Resistant Complexes without Caveolin. J Cell Biol., 1995, vol. 129(3), p. 619-627. GRIMMINGER, F., GRIMM, H., FUHRER, D., PAPAVASSILIS, C., LINDEMANN, G., BLECHER, C., MAYER, K., TABESCH, F., KRAMER, H. J., STEVENS, J., SEEGER, W. Omega-3 Lipid Infusion in a Heart Allotransplant Mo<strong>de</strong>l. Shift in Fatty Acid 176
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N° : 2006-ISAL-0031 ANNEE 2006 THE
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GOUTTE R. (Prof. émérite) CREATIS
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SIGLE ECOLE DOCTORALE NOM ET COORDO
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Dédicace Je dédie ce travail A me
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Je remercie également Mme Madelein
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AVANT-PROPOS Le travail présenté
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TABLE DES MATIERES REMERCIEMENTS...
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II-3-5-1 Huile d’olive...........
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IV-1-3-4 Distribution de la protéi
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Figure 26 : Gradient de saccharose
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Tableau XX : Effet des régimes sur
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MAP Kinase: Mitogen Activated Prote
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al., 1998 ; Steed et al., 1998). Le
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II- Rappels bibliographiques II-1-
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Figure 1 : Hématopoïèse du syst
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Tableau I : Antigènes couramment u
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Il existe cinq classes d’immunogl
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II-1-3-2-3 Les lymphocytes non T no
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Par la suite, de telles structures
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II-1-4-2 La composition des radeaux
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acylation (N-palmitoylation et S-my
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Tableau IV : Techniques d’analyse
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distingue d’une seconde zone de l
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essentiel du DAG est d’activer le
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R1 -O- R2 -O- Transphosphatidylatio
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II-2-4 Clonage et caractérisation
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Figure 8: Représentation schémati
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II-2-5-3 Les domaines PH et PX Il e
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Tableau VI: Caractéristiques des P
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Figure 10: La superfamille des prot
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Figure 11: la famille des PKC. En u
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phosphatidylinositol-4-monophosphat
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auteurs, PLD2 serait la forme préd
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prolifération, la croissance cellu
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II-3-2 Nomenclature des AG La nomen
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II-3-3 Les acides gras essentiels (
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II-3-4-3 Rôle fonctionnel II-3-4-3
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Tableau IX: Distribution (mole %) d
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II-3-5-1 Huile d’olive Le Maroc e
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L’analyse de la composition en AG
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1998; Kelley, 2001; Calder et al.,
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(1991) ont montré que les AGS ont
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uniquement chez les lymphocytes pro
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II-3-7-1 Effets des AG sur la synth
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II-3-7-2 Effets des AG sur les rade
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II-3-7-3 Effets des AG sur la struc
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Tableau XI : Influence des régimes
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III- MATERIELS ET METHODES III-1 Ma
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III-2 Méthodes III-2-1 Préparatio
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III-2-2 Préparation des échantill
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A B Figure 23: A : Métabolisme du
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III-2-5-4 Séparation du Pbut et du
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III-2-7 Analyse de la composition e
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croissantes (0.2M à 0.9M), puis l
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éalisée dans les mêmes condition
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vortexé avant d'être coulé. Apr
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Tableau XIII: Séquence des amorces
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IV- RESULTATS IV-1 Effet des acides
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Viabilité cellulaire (% du contrô
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métaboliquement stable et sa synth
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14 Activité PLD (pmoles de PC hydr
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L’ensemble des résultats concern
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- Page 131 and 132: IV-1-3-2 Détection du PIP2 Le PIP2
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- Page 153 and 154: V- Discussion De nombreuses études
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- Page 175: FOWLER, K. H., MCMURRAY, D. N., FAN
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- Page 189 and 190: MILES, E. A., CALDER, P. C. Modulat
- Page 191 and 192: NISHIZUKA, Y. The Molecular Heterog
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- Page 195 and 196: SCHERER, P. E., OKAMOTO, T., CHUN,
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- Page 199 and 200: TEITELBAUM, J. E., ALLAN WALKER, W.
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