ENDRES, S., GHORBANI, R., KELLEY, V. E., GEORGILIS, K., LONNEMANN, G., VAN DER MEER, J. W., CANNON, J. G., ROGERS, T. S., KLEMPNER, M. S., WEBER, P. C., ET AL. The Effect of Di<strong>et</strong>ary Supplementation with N-3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells. N Engl J Med., 1989, vol. 320(5), p. 265-271. ENDRES, S., MEYDANI, S. N., GHORBANI, R., SCHINDLER, R., DINARELLO, C. A. Di<strong>et</strong>ary Supplementation with N-3 Fatty Acids Suppresses Interleukin-2 Production and Mononuclear Cell Proliferation. J Leukoc Biol., 1993, vol. 54(6), p. 599-603. ENGLISH, D. Phosphatidic Acid: A Lipid Messenger Involved in Intracellular and Extracellular Signalling. Cell Signal., 1996, vol. 8(5), p. 341-347. ERICKSON, K. L., MCNEILL, C. J., GERSHWIN, M. E., OSSMANN, J. B. Influence of Di<strong>et</strong>ary Fat Concentration and Saturation on Immune Ontogeny in Mice. J Nutr., 1980, vol. 110(8), p. 1555-1572. EXTON, J.H. Phospholipase D: enzymology, mechanisms of regulation, and function. Physiol. Rev., 1997a, vol 77 (2), p. 303-320. EXTON, J.H. New <strong>de</strong>velopments in phospholipase D. J. Biol. Chem., 1997b, vol 272 (25), p. 15579-15582. EXTON, J. H. Regulation of Phospholipase D. FEBS L<strong>et</strong>t., 2002, vol. 531(1), p. 58-61. FERNANDO, K. C., GARGETT, C. E., WILEY, J. S. Activation of the P2z/P2x7 Receptor in Human Lymphocytes Produces a Delayed Permeability Lesion: Involvement of Phospholipase D. Arch Biochem Biophys., 1999, vol. 362(2), p. 197-202. FIELDING, C. J., FIELDING, P. E. Relationship b<strong>et</strong>ween Cholesterol Trafficking and Signaling in Rafts and Caveolae. Biochim Biophys Acta., 2003, vol. 1610(2), p. 219-228. FOSTER, D. A., XU, L. Phospholipase D in Cell Proliferation and Cancer. Mol Cancer Res., 2003, vol. 1(11), p. 789-800. 174
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N° : 2006-ISAL-0031 ANNEE 2006 THE
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GOUTTE R. (Prof. émérite) CREATIS
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SIGLE ECOLE DOCTORALE NOM ET COORDO
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Dédicace Je dédie ce travail A me
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Je remercie également Mme Madelein
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AVANT-PROPOS Le travail présenté
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TABLE DES MATIERES REMERCIEMENTS...
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II-3-5-1 Huile d’olive...........
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IV-1-3-4 Distribution de la protéi
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Figure 26 : Gradient de saccharose
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Tableau XX : Effet des régimes sur
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MAP Kinase: Mitogen Activated Prote
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al., 1998 ; Steed et al., 1998). Le
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II- Rappels bibliographiques II-1-
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Figure 1 : Hématopoïèse du syst
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Tableau I : Antigènes couramment u
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Il existe cinq classes d’immunogl
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II-1-3-2-3 Les lymphocytes non T no
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Par la suite, de telles structures
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II-1-4-2 La composition des radeaux
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acylation (N-palmitoylation et S-my
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Tableau IV : Techniques d’analyse
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distingue d’une seconde zone de l
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essentiel du DAG est d’activer le
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R1 -O- R2 -O- Transphosphatidylatio
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II-2-4 Clonage et caractérisation
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Figure 8: Représentation schémati
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II-2-5-3 Les domaines PH et PX Il e
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Tableau VI: Caractéristiques des P
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Figure 10: La superfamille des prot
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Figure 11: la famille des PKC. En u
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phosphatidylinositol-4-monophosphat
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auteurs, PLD2 serait la forme préd
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prolifération, la croissance cellu
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II-3-2 Nomenclature des AG La nomen
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II-3-3 Les acides gras essentiels (
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II-3-4-3 Rôle fonctionnel II-3-4-3
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Tableau IX: Distribution (mole %) d
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II-3-5-1 Huile d’olive Le Maroc e
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L’analyse de la composition en AG
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1998; Kelley, 2001; Calder et al.,
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(1991) ont montré que les AGS ont
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uniquement chez les lymphocytes pro
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II-3-7-1 Effets des AG sur la synth
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II-3-7-2 Effets des AG sur les rade
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II-3-7-3 Effets des AG sur la struc
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Tableau XI : Influence des régimes
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III- MATERIELS ET METHODES III-1 Ma
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III-2 Méthodes III-2-1 Préparatio
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III-2-2 Préparation des échantill
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A B Figure 23: A : Métabolisme du
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III-2-5-4 Séparation du Pbut et du
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III-2-7 Analyse de la composition e
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croissantes (0.2M à 0.9M), puis l
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éalisée dans les mêmes condition
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vortexé avant d'être coulé. Apr
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Tableau XIII: Séquence des amorces
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IV- RESULTATS IV-1 Effet des acides
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Viabilité cellulaire (% du contrô
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métaboliquement stable et sa synth
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14 Activité PLD (pmoles de PC hydr
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