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Introduction au RNA-seq - Institut de Mathématiques de Toulouse

Introduction au RNA-seq - Institut de Mathématiques de Toulouse

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Préparation ation <strong>de</strong>s librairies<br />

ies<br />

<br />

Purification <strong>de</strong>s ARNm sur billes magnétiques poly-T (s<strong>au</strong>f pour les bactéries)<br />

Liaison billes poly-T Séparation Lavages/élution<br />

Echantillon<br />

non traité<br />

Echantillon traité<br />

Pourcentage d'élimination i i <strong>de</strong>s ARNr<br />

90% 98% 99% 99,90%<br />

ARNr 98% 83% 50% 33% 5%<br />

Autres 2% 17% 50% 67% 95%<br />

Kits Illumina TruSeq<br />

<br />

Fragmentation chimique <strong>de</strong> l’ARN (tampon alcalin)<br />

http://get.genotoul.fr

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