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The Structural Analysis of Large Noncovalent Oxygen Binding Proteins Current Protein <strong>and</strong> Peptide Science, 2008, Vol. 9, No. 2 159<br />

Table 1.<br />

Experimental Subunit Masses, Native Masses, Calculated Masses <strong>and</strong> Architectural Features of Annelid HBL-Hbs<br />

Species<br />

Clitellata<br />

Hirudinida<br />

Erpobdellidae<br />

Nephelopsis<br />

oscura<br />

Number<br />

<strong>and</strong> type of<br />

subunits a<br />

Subunit Composition Dodecamer Mass Native Mass<br />

Expl mass<br />

(Da) ±s.d.<br />

Subunits from<br />

covalent<br />

complexes b<br />

Expl mass<br />

(Da) ±s.d.<br />

Expl mass<br />

(Da) ±s.d.<br />

[reference]<br />

Cltd mass<br />

(model)<br />

Expl mass<br />

(kDa) ±s.d.<br />

Method c<br />

[reference]<br />

Calculated<br />

Mass (kDa)<br />

144G + 36L<br />

model<br />

[reference]<br />

3D Structure<br />

type I/II p <strong>and</strong><br />

central piece<br />

[reference]<br />

3 MG 16534.5 ±1 208670 ±40 208330 nd 3398 nd<br />

17170.5 ±1 6M + 3D [144]<br />

(Hb) 17314.7 ±1 [144] ESI-MS data<br />

2 DG 32949.5 ±3 3 G < DG 16321.8 ±1<br />

33125.4 ±3 16498.6 ±1<br />

16632.4 ±1<br />

5 ML 24512.3 ±2<br />

24585.5 ±2<br />

24978.9 ±2<br />

25005.8 ±2<br />

25565.8 ±2<br />

[144]<br />

Haemopidae<br />

Haemopis gr<strong>and</strong>is 4 MG 16634,1 ±0,5 207550 ±191 207437 3710 d SE 3361 nd<br />

17013,4 ±0,5 6M + 3D [146] [32]<br />

(Hb) 17592,9 ±0,5 [63] ESI-MS data<br />

17573,3 ±0,8<br />

2 DG 32501,1 ±0,7 3 G < DG 16100,4 ±0,5<br />

32629,6 ±0,8 16405,8 ±0,4<br />

16705,2 ±0,5<br />

3 ML 24004,2 ±0,4<br />

24449,2 ±1,1<br />

24548,9 ±0,6<br />

[145]<br />

Hirudinidae<br />

Macrobdella<br />

decora<br />

2 MG 16770,1 ±0,2 206528 ±16 206371 3560 ±160 STEM 3357 e type I<br />

16841,9 ±0,5 6M + 3D [147] toroid<br />

(Hb) 3 DG 32586 ±1,1 4 G < DG 16052,2 ±0,5 [63] 3804 ±70 MALLS [149]<br />

32714,5 ±0,8 16537,3 ±0,6 [148]<br />

32842,9 ±1,3 16666,7 ±1<br />

16792,9 ±0,6<br />

3 ML 24340,1 ±0,7<br />

24398,6 ±0,3<br />

24420 ±0,4<br />

[82]<br />

Oligochaeta<br />

Glossoscolecidae<br />

Glossoscolex<br />

paulistus<br />

4 MG 16372 ±18 212833,5 e 3100 SV 3500 e<br />

16447 ±19 3M + 3T [151]<br />

(Hb) 16613 ±7 3200 SAXS<br />

16750 ±70 [152]<br />

1 DG 32520 f<br />

1 TG 51933 3 G < TG 16492 ±24<br />

17363 ±17<br />

18258 ±30<br />

3 ML 25817 ±50<br />

26761 ±16<br />

34130 g<br />

[150]<br />

Lumbricidae<br />

Lumbricus<br />

terrestris<br />

3 MG 15992,4 ±1 213593 ±203 213436 3560 ±130 STEM 3531 type I<br />

15978 ±1 3M + 3T [23] [23] toroid<br />

(Hb) 15962,1 ±1 [63] 3410-3660 SE ESI-MS data [153]<br />

4 TG 52922,6 6 G < TG 16254,4 ±1 [23] 3516 ±16<br />

52760 17289,2 ±1 3755 ±80 MALLS [32]<br />

52598,5 19389,9 ±1 h [148] statistical model<br />

3 ML 27607 i<br />

52435,4 19227,4 ±1 h 4100 ±100 MALLS<br />

32104,3 ±5<br />

24912,9 ±2<br />

24090 j<br />

[23]<br />

19065,3 ±1 h [22]<br />

19065,3 ±2 h<br />

80

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