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122 CHAPITRE 3. STRUCTURE DE L’HC DE C. MAENAS PAR ESI-MS FIG. 3.6 – Acidic reassociation of Carcinus maenas hemocyanin by lactic acid. Left part, first spectrum, mass spectrum of a whole hemolymph sample dissociated in 10 mM AcNH4, 0.03 % TEA. Second and third mass spectra, mass spectra of the dissociated hemocyanin treated with 2mM lactic acid and with or without addition of a further 0.04 % TEA (pH 8.58 and 5.89, respectively). m, monomers peaks, h, hexamer peaks (450 kDa), d, dodecamer peaks (900 kDa), dl, light dodecamer peaks (885 kDa). Right part, focus for each mass spectrum on the monomer peaks m/z range.
3.2. MANUSCRIT : STRUCTURAL STUDY OF C. MAENAS HC BY ESI-MS 123 FIG. 3.7 – Alkaline dissociation and formic acid reassociation of purified Carcinus maenas hemocyanin dodecamers and hexamers. Left part, mass spectra for purified dodecamers. Right part, mass spectra for purified hexamers. First row, purified complexes in 10 mM AcNH4, pH 6.66. Second row, complexes dissociated by addition of 0.03 % TEA (pH 8.55). Third row, complexes reassociated by addition of 0.0045 % formic acid (pH 7.7). Fourth row, complexes dissociated anew by the addition of formic acid to a final concentration of 0.065 % (pH
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THESE DE DOCTORAT DE L’UNIVERSITE
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i Résumé / Abstract Réponse adap
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iv SOMMAIRE 4.2 Objectifs de l’é
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2 CHAPITRE 1. INTRODUCTION Les éco
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4 CHAPITRE 1. INTRODUCTION 1.1 Mili
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6 CHAPITRE 1. INTRODUCTION Les prin
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8 CHAPITRE 1. INTRODUCTION TAB. 1.1
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10 CHAPITRE 1. INTRODUCTION et cycl
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FIG. 1.5 - Localisation des princip
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(a) FIG. 1.6 - Fonctionnement d’u
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16 CHAPITRE 1. INTRODUCTION TAB. 1.
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18 CHAPITRE 1. INTRODUCTION FIG. 1.
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20 CHAPITRE 1. INTRODUCTION Différ
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24 CHAPITRE 1. INTRODUCTION le mili
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26 CHAPITRE 1. INTRODUCTION Capacit
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28 CHAPITRE 1. INTRODUCTION TAB. 1.
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30 CHAPITRE 1. INTRODUCTION 700 600
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32 CHAPITRE 1. INTRODUCTION férent
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34 CHAPITRE 1. INTRODUCTION (a) (b)
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40 CHAPITRE 1. INTRODUCTION 1 P 50
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42 CHAPITRE 1. INTRODUCTION presque
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44 CHAPITRE 1. INTRODUCTION affecte
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50 CHAPITRE 1. INTRODUCTION fure co
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52 CHAPITRE 1. INTRODUCTION amélio
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54 CHAPITRE 1. INTRODUCTION 1.5 Que
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56 CHAPITRE 2. ESI-MS ET MALLS APPL
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Page 78 and 79: The Structural Analysis of Large No
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172 CHAPITRE 5. ADAPTATIONS RESPIRA
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216 CHAPITRE 6. CONCLUSION ET PERSP
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Annexe A Détermination des masses
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229 10000 h280 .M h) h280−h337 80
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Annexe B Détermination des distrib
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233 Modèle 2 : 2 sous-unités lég
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235 1 0.9 0.8 6 s-u aléatoires 2 s
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Bibliographie Adair, G. S. The hemo
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BIBLIOGRAPHIE 239 Catalina, M. I.,
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BIBLIOGRAPHIE 241 Farmer, C. S., J.
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BIBLIOGRAPHIE 243 Hourdez, S. Adapt
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BIBLIOGRAPHIE 245 Mangum, C. P. Sub
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BIBLIOGRAPHIE 247 Rainer, J., C. P.
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BIBLIOGRAPHIE 249 Svedberg, T. et I
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BIBLIOGRAPHIE 251 Vanin, S., E. Neg
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Liste des figures 1.1 Cycle des mar
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LISTE DES FIGURES 255 4.17 Profils
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Table des matières Résumé Sommai
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TABLE DES MATIÈRES 261 4.3.5 Spect
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TABLE DES MATIÈRES 263 Table des m
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