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maenas (intertidal zone) and Segonzacia mesatlantica - Station ...

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3.2. MANUSCRIT : STRUCTURAL STUDY OF C. MAENAS HC BY ESI-MS 109<br />

Structural study of Carcinus <strong>maenas</strong> hemocyanin by supramolecular<br />

ESI-MS : interaction with L-lactate <strong>and</strong> divalent cations<br />

Matthieu Bruneaux 1,2 , Peran Terrier 3,4 , Emmanuelle Leize 3 , Jean Mary 1,2 , François H. Lallier 1,2 ,<br />

Franck Zal 1,2 *<br />

1 UPMC Univ. Paris 06, UMR 7144, Equipe Ecophysiologie : Adaptation et Evolution Moléculaires,<br />

<strong>Station</strong> Biologique de Roscoff, 29682 Roscoff, France<br />

2 CNRS, UMR 7144, <strong>Station</strong> Biologique de Roscoff, 29682 Roscoff, France<br />

3 CNRS-ULP, UMR 7177, Laboratoire de Dynamique et Structure Moléculaire par Spectrométrie<br />

de Masse, Institut de Chimie, ISIS, 67083 Strasbourg, France<br />

4 CNRS-UEVE, UMR 8587, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement,<br />

Université d’Evry-Val d’Essonne, 91025 Evry, France<br />

M.B. was funded by a MRT grant, n°18213-2005. P.T. was funded by an ANR grant, n°ANR-05-<br />

MIIM-030-03 <strong>and</strong> an ATER grant from UEVE.<br />

*Corresponding Author : F. Zal, <strong>Station</strong> Biologique de Roscoff, Place Georges Teissier, BP74,<br />

29682 Roscoff cedex, FRANCE Phone : 0033 (0)298292309 Fax : 0033 (0)298292324 E-mail :<br />

zal@sb-roscoff.fr<br />

Running title : Structural study of C. <strong>maenas</strong> hemocyanin by supramolecular ESI-MS<br />

Abbreviations : AcNH4, ammonium acetate ; DPG, D-2,3-diphosphoglycerate ; ESI-MS, electrospray<br />

ionization mass spectrometry ; FA, formic acid ; MWC model, Monod-Wyman-Changeux<br />

model ; SEC, size-exclusion chromatography ; TEA, triethanolamine

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