caractérisation des enzymes bovines et étude préliminaire du rôle ...

Table II. Primers used to amplify and sequence bovine Pofut1 and Pofut2 genes.
Pofut1 Primers Sequences
BosPofut1/5’end 5’-ATGCATGGGCAGTAGAGC-3’
Intron 1/5’ 5’-GCGCTCTGGCACTGGACCCG-3’
Intron 2/5’ 5’-CGTTTTGGGAACCAGGCTGATCATTTC-3’
Intron 2/3’ 5’-GGTGCCAGCTTCTCCATGAAGTCCTCC-3’
Intron 3/5’ 5’-TCCTACCAGAAGTACTTCAAGCTGGAG-3’
Intron 3/3’ 5’-ATCCACTGGTCTTTGTAGGAGGCACTG-3’
Intron 4/5’ 5’-GGCCCATTTTGGGATCAGTTTCATGTG-3’
Intron 4/3’ 5’-GGATCTGGATCAGCTCACAC-3’
Intron 5/5’ 5’-CATTCCCCACTTTCACTCCAA-3’
Intron 5/3’ 5’-CCCCTGTCCTCACCATCTCGTCTGACC-3’
Intron 6/5’ 5’-CAGAGGAACATCCAGTGCTTG-3’
Intron 6/3’ 5’-TTTGAAGAGCTGCTGGATCTC-3’
Intron 7/5’ 5’-ACGCGTGTGCCATGCTGAAAG-3’
Intron 7/3’ 5’-CCTGTCCATGCCAAAGAAGGAGG-3’
Intron 8/5’ 5’-GAAGGTGGTGAGTCTGAAGCC-3’
Intron 8/3’ 5’-CGAGCAGCAAGGACTACTCTG-3’
BosPofut1/3’end 5’-AGCCGAGTTTTGCTGTTCCGAAG-3’
Pofut2 Primers Sequences
BosPofut2/5’end 5’-GCGATGAACTGCTCGTACTC-3’
Intron 1/5’ 5’-TTGCCGTCGTCTTCCTGCTG-3’
Intron 2/5’ 5’-CCTTCTGTACGATGTCAATCC-3’
Intron 2/3’ 5’-GTAATACTCGTGCTTGTCCTG-3’
Intron 3/5’ 5’-CAGGACAAGCACGAGTATTAC-3’
Intron 3/3’ 5’-TTCGTAACCCCAAAACCATCC-3’
Intron 4/5’ 5’-TGGTTTTGGGGTTACGAAGAG-3’
Intron 4/3’ 5’-TACTCCTTCCCGCCGTAGTGG-3’
Intron 5/5’ 5’-GAGGAGGACTGGACCCGGATG-3’
Intron 5/3’ 5’-CCCAGCGAGGAGCCCAGCTTG-3’
Intron 6/5’ 5’-AGCATGGTGTTCGCCAGGCAC-3’
Intron 6/3’ 5’-ACAGCATCTGTGGCCACGAAC-3’
Intron 7/5’ 5’-CAAGCTGGGCTCCTCGCTGGG-3’
Intron 7/3’ 5’-CGGGCGTGCGCACAGATCCAC-3’
Intron 8/5’ 5’-AGGAGTTGCGGAGGCTGCTGC-3’
Intron 8/3’ 5’-AGTATGCAATCCTCCAATGAGTGG-3’
BosPofut2/3’end 5’-TCATTGGAGGATTGCATACTG-3’
128