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International Journal of Mediterranean Ecology - Ecologia ...

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ADEL DHIEF, MUSTAPHA GORAI, SAMIRA ASCHI-SMITI, MOHAMED NEFFATI<br />

Germination (%)<br />

Figure 1 – Germination percentage <strong>of</strong> three<br />

Calligonum species after different<br />

pre-sowing seed treatments.<br />

22<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

C. comosum<br />

0 2 4 6 8 10 12 14 16<br />

C. azel<br />

0 2 4 6 8 10 12 14 16<br />

C. arich<br />

0 2 4 6 8 10 12 14 16<br />

Days after sowing<br />

C T1 T2 T3 T4 T5 T6<br />

C: untreated seeds (•); T1: seeds grinded in a mortar<br />

with a pinch <strong>of</strong> clean silica sand (); T2: seeds rubbed<br />

with sandpaper (); T3: seeds soaked in hot water<br />

(); T4, T5 and T6: seeds immersed in sulphuric acid<br />

for 10 (), 20 () and 30 min (*), respectively. The<br />

symbols are the observed numbers <strong>of</strong> germinated<br />

seeds, expressed as percent <strong>of</strong> sown seeds (n = 4).<br />

Figure 3 – Changes in the index <strong>of</strong> germination rate<br />

<strong>of</strong> three Calligonum species during 30 d<br />

after different pre-sowing seed<br />

treatments (Dhief et al.).<br />

See Figure 2 for legend.<br />

(C): untreated seeds (control); (T1): mechanical scarification by grinding<br />

seeds in a mortar with a pinch <strong>of</strong> clean silica sand; (T2): mechanical<br />

scarification by rubbing seeds with sandpaper; (T3): immersion in boiling<br />

water for 10 min; (T4): immersion in concentrated sulphuric acid (96%) for<br />

10 min; (T5): immersion in concentrated sulphuric acid for 20 min; (T6):<br />

immersion in concentrated sulphuric acid for 30 min. Data represent mean<br />

± SE (n = 4) and different letters indicate significant differences between<br />

treatments at p < 0.05 (Duncan test).<br />

Figure 2 – Changes in the final seed germination (a) and seed relative<br />

germination percentages (b) <strong>of</strong> three Calligonum species during<br />

30 d after different pre-sowing seed treatments (Dhief et al.).<br />

ecologia mediterranea – Vol. 38 (1) – 2012

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