UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

More documents

Recommendations

Info

Résultats et discussion. Chapitre 2: Optimisation de la production du spawn The substrates used for L. edodes growth in both liquid and solid state culture exhibited constant ergosterol content in mycelium. Mean value of ergosterol content in pure mycelium 3.57± 0.56 (mg.g-1 of dry weight) was within the range found in other experiments with other microorganism (Pasanen et al., 1999; Barajas-Aceves 2000; Jasinghe and Perera 2005). While some of fungal species exhibited large differences in ergosterol content between the isolates, as can be seen from the relatively large standard deviation (Klamer and Bååth 2004; Mille- Lindblom et al., 2004; De Ridder-Duine et al., 2006). It would appear that culturing of mycelium on EbA could allow for operating nearly to natural condition so as to gain insight into the magnitude of variation and hence the potential error when calculating fungal biomass from ergosterol concentration measured in spawn fermentation (Gessner and Chauvet 1993). Determination of ergosterol allowed for reducing the use of hazardous organic solvents which involved in saponification step of ergosteryl ester (De Ridder-Duine et al., 2006), and this thereby permeates for measuring of living fungal biomass (Mille-Lindblom et al., 2004) based on the assumption that the ergosterol have been considered as kind of lipids storage (Klamer and Bååth 2004). Furthermore, the recovery of ergosterol with this simple protocol was exhibited in the most of case very high as could be reported by several authors (Gong et al., 2001; De Ridder-Duine et al., 2006; Yuan et al., 2007). The identification of ergosterol with HPLC was achieved by comparing its retention time and spectrum against ergosterol standard (Fig. 6). The ergosterol spectrum exhibited characteristic pattern, because it has a conjugated pair of double bonds at carbons 5-6 and 7-8, it absorbs UV light strongly between 240 and 300 nm. The interference of sterol containing in wheat, as reported by Seitz et al. (1977), was eliminated by verification of purity of ergosterol peak detected by HPLC, and verification of spectrum of peak corresponding to ergosterol. The conversion of ergosterol, which was considered as precursor, to vitamin D2 (Ergocalciferol) by UV irradiation was reported by several authors (Jasinghe and Perera 2005; Jasinghe and Perera 2006; Teichmann et al., 2007). Certain author investigated conversion kinetics of ergosterol to vitamin D2 in natural products such as in L. edodes mushrooms so as to modeling of conversion in order to predict the yield of vitamin D2 after the irradiation process. 85
D.O (mAU) Résultats et discussion. Chapitre 2: Optimisation de la production du spawn Figure 6. UV spectrum (260- 300 nm) of ergosterol extracted from mycelium of L. edodes Le119 strain grown on Ebly ® in solid state fermentation. 5. Conclusion The results presented in this paper showed that the non-esterified ergosterol measurement of L. edodes can be considered as a reliable indirect method for estimating fungal biomass present in spawn. In fact, non-esterified ergosterol content is constant whatever the age of the mycelium, culture conditions and medium composition. In the other hand, this study demonstrated that the established correlations between Ergosterol and CO2 rate production in solid state fermentation permit a good estimation of the physiological state of mycelial biomass. By using the linear regression equation obtained in solid state culture, quantities of non-esterified ergosterol measured could be easily converted into fungal dry matter quantities per gram of dry solid medium (1 g of mycelial biomass contain 3.57 mg of ergosterol). To optimize a solid state fermentation process for biomass production, the use of such method cannot be avoided. Wavelength (nm) 86
Page 1 and 2:
UNIVERSITÉ PAUL CÉZANNE, AIX MARS
Page 3 and 4:
Je remercie le Dr. Antonis PHILIPPO
Page 5 and 6:
Résumé L’objectif du travail a
Page 7 and 8:
2.2.4.2. Culture de champignons sup
Page 9 and 10:
3.7.1. La mesure de l’humidité .
Page 11 and 12:
Liste des Tables Tableau 1. Bilan m
Page 14 and 15:
1. INTRODUCTION Introduction Au Mar
Page 16:
Introduction L'utilisation de la fe
Page 19 and 20:
Matériel et Méthodes Figure 2. Ev
Page 21 and 22:
Matériel et Méthodes et de l’hu
Page 23 and 24:
Matériel et Méthodes composition
Page 25 and 26:
Matériel et Méthodes Il ressort d
Page 27 and 28:
Matériel et Méthodes catéchol-m
Page 29 and 30:
Matériel et Méthodes Pour une év
Page 31 and 32:
Matériel et Méthodes nombreuses o
Page 33 and 34:
Matériel et Méthodes agricoles (P
Page 35 and 36:
Matériel et Méthodes b) Traitemen
Page 37 and 38:
Matériel et Méthodes pour former
Page 39 and 40:
Matériel et Méthodes (protéines,
Page 41 and 42:
2.4.2.5. Contrôle de la températu
Page 43 and 44:
2.4.4. Les avantages et les inconv
Page 45 and 46:
Matériel et Méthodes 32
Page 47 and 48: Figure 9. Unité de trituration sem
Page 49 and 50: 3.3. Les milieux de culture 3.3.1.
Page 51 and 52: Matériel et Méthodes Mesure du po
Page 53 and 54: 3.5.4. La régulation des paramètr
Page 55 and 56: Matériel et Méthodes 0 et 10%. Ce
Page 57 and 58: Matériel et Méthodes méthanol gr
Page 59 and 60: Matériel et Méthodes gallique (1
Page 61 and 62: Matériel et Méthodes Tableau 11 .
Page 63 and 64: Résultats et discussion La partie
Page 65 and 66: Résultats et discussion. Chapitre
Page 69 and 70: 1. Introduction Résultats et discu
Page 75 and 76: 3.2. Biomass production Résultats
Page 93 and 94: 3. Results Résultats et discussion
Page 95 and 96: Ergosterol (mg) Résultats et discu
Page 97: Ergosterol content (mg.g -1 ) Résu
Page 127 and 128: Biomasse (mg.g -1 SPS) Résultats e
Page 129 and 130: mg.g -1 SPS du substrat fermenté 4
Page 141 and 142: ABSTRACT Résultats et discussion.
Page 147 and 148: 3. Results and discussion Résultat
Page 149 and 150:
Résultats et discussion. Chapitre
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Synthèse générale 142
Page 157 and 158:
Synthèse générale produits de l
Page 159 and 160:
Synthèse générale Suite à l’o
Page 161 and 162:
Synthèse générale molécules ren
Page 163 and 164:
Conclusions et perspectives 150
Page 165 and 166:
Conclusions et perspectives La mise
Page 167 and 168:
Références bibliographiques Réf
Page 169 and 170:
Références bibliographiques Bing
Page 171 and 172:
Références bibliographiques D'Ann
Page 173 and 174:
Références bibliographiques mill
Page 175 and 176:
Références bibliographiques Jo J.
Page 177 and 178:
Références bibliographiques Match
Page 179 and 180:
Références bibliographiques Pietr
Page 181 and 182:
Références bibliographiques Sebab
Page 183 and 184:
Références bibliographiques Zerva
show all

UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?