UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP
UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP
UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP
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Ergosterol (mg)<br />
Résultats et discussion. Chapitre 2: Optimisation de la production du spawn<br />
0,2<br />
0,16<br />
0,12<br />
0,08<br />
0,04<br />
0<br />
y = 0,003x - 0,000<br />
R² = 0,999<br />
0 10 20 30 40 50<br />
Figure 3. Relationship between fungal dry weight and ergeosterol content, for L. edodes<br />
Le119 cultivated in solid state culture with cellophane system, incubated during 20 days at<br />
25°C.<br />
3.3. Recovery of spiked ergosterol:<br />
The recovery of methanol-dissolved ergosterol from spawn was estimated to 75-80%<br />
for both in brown and white spawn respectively, and the recovered value did not differ<br />
significantly (Table 2). However, 90% of recovery of fungal ergosterol was found for Le119<br />
strain.<br />
Biomass dry weight (mg)<br />
Table 2. Recovery of Sigma pure ergosterol and fungal ergosterol (homogenized<br />
mycelium of L. edodes Le119) added to Brown and White spawn<br />
Samples Recovery of pure<br />
ergosterol µg.g -1<br />
Brown Spawn 7 .52 3.17a 1<br />
White spawn 8.04 3.21a<br />
Added amount 2 10.0 3.55b<br />
Recovery of fungal<br />
ergosterol<br />
1 Different letters indicate significant differences.<br />
2 Added amount of ergosterol for Le119 was based on direct measurements of ergosterol in<br />
the mycelium using described protocol for extraction.<br />
82
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