UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

ABSTRACT
Résultats et discussion. Chapitre 2: Optimisation de la production du spawn
Edible and Medicinal Mushroom production is nowadays under huge expansion owing
to extensive demand in world Market. Thereby, mushroom culture is becoming more popular
throughout the world because of the improvement of mushroom culture utilizing various
lignocellulosic substrates with good yield of production. However, mushroom culture
successful depends primarily to quality, physiological state and size of spawn used. While the
quantification of spawn (mycelium produced on cereal grain) is very difficult to obtain. In the
present work, the determination of mycelial biomass of L. edodes spawn on wheat grain
Ebly® during spawn running is reported for the first time. Ergosterol of Le119 L. edodes
strain mycelium was measured. Non-Alkaline extractions were performed with ethanol at 4°C
for overnight. The extracted ergosterol was quantified by RP-HPLC against ergosterol pure as
standard. Effect of different substrates and ages of L. edodes mycelium were evaluated. No
statistical differences were exhibited for the Lentinula edodes Le119 strain. Presence of
supplement on wheat grain as olive mill wastewater in 10% (v/v) exhibited enhancement of
mycelial biomass yield of L. edodes strain. Furthermore, the respirometry (CO2 production) of
L. edodes cultivated in solid state fermentation on wheat grain during spawn running was
correlated to ergosterol content of fermented products (r 2 = 0.80).
Keywords: L. edode, Ergosterol, Spawn, Fungal Biomass, HPLC, Respirometry, Solid State
Fermentation, Conversion Factor.
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