UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

Résultats et discussion. Chapitre 1 : Criblage des souches de L. edodes
3.1. Tolerance of OMW by Shiitake strains
All strains of L. edodes were capable of growing on PDA with 10% and 20% of OMW
(v/v), while no mycelial growth was observed above 40% of OMW. The period of initial
growth varied among strains. By contrast, the period for complete colonization of the Petri
plate by L. edodes strains varied from 10-30 days. Apical growth rates were significantly
different among strains. They were reduced by 50% as the concentration of OMW increased
in PDA (Fig. 1).
Apical growth ( mm day -1 )
12,00
10,00
8,00
6,00
4,00
2,00
0,00
118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133
L. edodes strains
Figure 1: Growth rate (mm day -1 ) of sixteen L. edodes strains on PDA with 10% and 20% of
OMW in solid culture at 25°C until complete colonization of the Petri plate (diam. 90mm).
The apical growth of all strains was higher on PDA without OMW (data not shown).
The highest growth rate was recorded for Le118 growing on agar medium containing 10%
and 20% of OMW (10.72 and 4.97 mm day -1 , respectively), while the lowest growth rate was
shown by the strain Le126 (1.38 and 0.46 mm day -1 , respectively).
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