UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

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Résultats et discussion. Chapitre 1 : Criblage des souches de L. edodes manganese peroxidase and laccase (D’Annibale et al., 2004; Ayed et al., 2005; Matos et al., 2007). However, the production of these enzymes depends on substrate composition, cultivation system and environmental factors affecting fungal growth (Alaoui et al., 2008; Elisashvili et al., 2008). In previous research work, it has been shown that mycelial growth rate, biomass yield and enzymatic activities are important factors for successful mushroom cultivation (Philippoussis et al., 2003; Silva et al., 2005). In this study, we selected strains of L. edodes capable not only of tolerating the phenolics present in OMW, but also of using them as a carbon source to yield high mycelial biomass. Decolorization and removal of phenolic compounds were also criteria of selection. 2. Materials and methods 2.1. Substrates OMW was obtained from an olive oil plant at Beni-Mellal (central Morocco), after the three-phase extraction. OMW was stored at -20°C until used. The composition of untreated OMW is such as: pH (4.9), suspended solids (13.84 %), total sugar (12,8 g l-1), reducing sugar (10.08 g l-1) and total phenol (12.54 g l-1). 2.2. Microorganisms Sixteen strains of Lentinula edodes (Berk.) Pegler were obtained from different laboratories (Table 1). These strains were already selected for their ability to colonize agricultural residues. All strains were routinely grown on potato dextrose agar (PDA, Sigma, St Quentin Fallavier, France) plates. Inoculum was prepared by growing the mycelium on agar plates for 2 weeks. 57
Résultats et discussion. Chapitre 1 : Criblage des souches de L. edodes Table 1: Origin and source of different strains of Lentinula edodes Strains Origin Source Le118 AMRL* China Le119 AMRL China Le120 AMRL China Le121 AMRL Europe Le122 AMRL Europe Le123 AMRL Europe Le124 AMRL Taïwan Le125 Commercial strain Osaka Japon Le126 Commercial strain Osaka Japon Le127 Commercial strain France Mycelium Le128 Commercial strain France Le Champion Le129 Commercial strain Belgique Mycelia Le130 Commercial strain USA, Lamber spawn Le131 Commercial strain Japon Le132 Hybrid strain INRA France Le133 Homocaryon of commercial strain Le Lion France * Athens Mushroom Research Laboratory (NAGREF) 2.3. Solid State Culture Tolerance of shiitake mycelium was investigated on PDA containing different concentrations of OMW, ranging from 0-100%. The agar medium was sterilized at 121°C for 20 min, cooled, and used to prepare PDA plates. These plates were inoculated with agar disks (10 mm diameter) from an actively growing colony, and then incubated at 25°C. Linear growth rates were determined as the average distance covered by the mycelium in two perpendicular directions. After complete colonization of Petri plates (90 mm diameter), the mycelial biomass was assessed using a sterile cellophane disc placed on the agar surface, as described by De Araujo et al. (2000). The mycelium was allowed to grow on the cellophane disc, and then lifted away to be dried at 105°C. 58
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UNIVERSITÉ PAUL CÉZANNE, AIX MARS
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Je remercie le Dr. Antonis PHILIPPO
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Résumé L’objectif du travail a
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2.2.4.2. Culture de champignons sup
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3.7.1. La mesure de l’humidité .
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Liste des Tables Tableau 1. Bilan m
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1. INTRODUCTION Introduction Au Mar
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Introduction L'utilisation de la fe
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1. Introduction Résultats et discu
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Résultats et discussion. Chapitre
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Biomasse (mg.g -1 SPS) Résultats e
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mg.g -1 SPS du substrat fermenté 4
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ABSTRACT Résultats et discussion.
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3. Results and discussion Résultat
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Synthèse générale 142
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Synthèse générale produits de l
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Synthèse générale Suite à l’o
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Synthèse générale molécules ren
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Conclusions et perspectives 150
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Conclusions et perspectives La mise
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Références bibliographiques Réf
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Références bibliographiques Bing
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Références bibliographiques D'Ann
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Références bibliographiques mill
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Références bibliographiques Jo J.
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Références bibliographiques Match
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Références bibliographiques Pietr
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Références bibliographiques Sebab
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Références bibliographiques Zerva
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UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

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