UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP
UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP
UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP
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ABSTRACT<br />
Résultats et discussion. Chapitre 1 : Criblage des souches de L. edodes<br />
In Morocco, olive industry produces great amounts of Olive Mill Wastewater (OMW)<br />
in short time period (250 000 m 3 of liquid wastes in four months, November-February).<br />
Phenolic compounds are largely responsible for the phytotoxicity and antimicrobial effects of<br />
OMW. Several studies have been carried out focusing on the biological and enzymatic<br />
treatment of OMW. However, the use of OMW to produce value-added products, e.g.<br />
mushroom cultivation, are less explored. This research aimed to select shiitake mushroom,<br />
capable of growing on OMW, involving decolorization, removal of total phenol and high<br />
production of mycelial biomass. Sixteen strains of Lentinula edodes were evaluated for their<br />
tolerance to OMW, apical growth rate and biomass production on agar media. The highest<br />
biomass yields were recorded in four L. edodes strains (Le118, Le119, Le121 and Le122)<br />
grown in the presence of 20% (v/v) OMW. The ability of these pre-selected strains to<br />
decolorize and to remove total phenol from OMW was then tested by using the technique of<br />
liquid culturing, without any other nutriment supplement. L. edodes strain Le119 exhibited<br />
65% decolorization along with 75% elimination of total phenol as resulted from Folin<br />
Ciocalteu assays. The laccase activity was the main lignolytic activity observed, with one<br />
isoform of the enzyme got stained on native PAGE with p-phenylenediamine as the substrate<br />
at pH 5.0.<br />
Keywords: Lentinula edodes, Olive Mill Wastewater, Solid State Culture, Liquid State<br />
Culture, Biomass, Total Phenol, Laccase, Detoxification.<br />
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