UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

Résultats et discussion. Chapitre 3: Evaluation des facteurs de croissance
From Figure 1 and Table 8a was observed that the significant parameter effects were
mineral solution (F) and inoculum size (D), while the rest of the parameters had no significant
effects. Figure 1a showed that inoculum size exhibited significant positive effects on the
response: the increase of these variables resulted in the increase of mycelial biomass which
was correlated to the total phenol removal. The mineral solution, on the other hand, exhibited
a negative effect on the response. Finally, the use of OMW without supplements showed an
interesting positive effect on the mycelial biomass (Fig. 1a).
3.5. Variable effects on total phenol removal response
Coefficient values and related statistical analyses are reported in Table 8b. These results
are illustrated by the two histograms shown in Figure 2. The significant parameter effects
were found, at all levels studied, as mineral solution (F) and inoculum size (D). Initial pH of
substrate, inoculum age, particle size and aeration seemed to have no significant effects. The
results showed a correlation between the parameters effecting the mycelial biomass and total
phenol removal.
3.6. Substrate composition
Measurements of pH and of different experiments after the colonization process by
L. edodes Le119 were different. A steady decrease of pH in all media without supplement
tested was observed; the initial pH values (5.0–6.0) fell at the end of incubation to 4.39 and
4.66 for experiment 1 and 16 respectively. The initial pH of substrates affected slightly the
final pH after the colonization. On the other hand, the supplemented media showed an
increase of pH after incubation (from 5 to 5.6 for experiment 9).
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