UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

3.3. Screening design
Résultats et discussion. Chapitre 3: Evaluation des facteurs de croissance
Sixteen experiments have been carried out according to the preparation method
described above and the conditions fixed by the experimental design. Although the main
purpose was to achieve the maximization of mycelial biomass production and the
maximization of total phenol removal. The obtained values related to the mycelia biomass
(mg of mycelium/g of substrate) and total phenol removal (%) are as shown in Table7.
Table 7. Experimental conditions of the screening design with 6 factors (A-F) and the
corresponding experimental responses.
Run
no
A B C D E F Biomass
Yield (mg.g -1 )
Total phenol
removal (%)
1 5 20 25 20 T2 SO 48.72 82.35
2 6 30 10 10 T1 SO 20.30 41.28
3 5 20 25 20 T1 MS1 34.50 61.65
4 6 30 10 10 T2 MS1 16.24 42.00
5 5 20 10 10 T2 MS3 12.18 24.12
6 6 30 25 20 T1 MS3 33.50 51.91
7 5 20 10 10 T1 MS2 29.44 36.23
8 6 30 25 20 T2 MS2 38.56 63.75
9 5 30 25 10 T2 MS4 12.18 15.48
10 6 20 10 20 T1 MS4 22.32 29.49
11 5 30 25 10 T1 MS3 14.20 31.51
12 6 20 10 20 T2 MS3 28.42 47.20
13 5 30 10 20 T2 MS1 34.50 45.45
14 6 20 25 10 T1 MS1 18.26 40.71
15 5 30 10 20 T1 SO 44.66 82.68
16 6 20 25 10 T2 SO 30.44 47.98
3.4. Variable effects on mycelial biomass response
Coefficient values (the weight associated to each factor level) and statistical analyses,
using t-test are reported in Table 8a. These results are illustrated by the two histograms shown
in Fig.1. Fig. 1a represents the weight of each factor taking its highest level as reference. Fig.
1b represents the differential effects of each factor when considering two different levels
taken two by two (Mathieu and Phan-Than-Luu 1997; Lewis et al., 1999).
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