UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

Résultats et discussion. Chapitre 3: Evaluation des facteurs de croissance
Table 4. Experimental conditions of the screening design with 6 factors (A-F).
Run No A B C D E F
1 5 20 25 20 T2 SO
2 6 30 10 10 T1 SO
3 5 20 25 20 T1 MS1
4 6 30 10 10 T2 MS1
5 5 20 10 10 T2 MS3
6 6 30 25 20 T1 MS3
7 5 20 10 10 T1 MS2
8 6 30 25 20 T2 MS2
9 5 30 25 10 T2 MS4
10 6 20 10 20 T1 MS4
11 5 30 25 10 T1 MS3
12 6 20 10 20 T2 MS3
13 5 30 10 20 T2 MS1
14 6 20 25 10 T1 MS1
15 5 30 10 20 T1 SO
16 6 20 25 10 T2 SO
2.5. Methods of analysis
2.5.1. Standard chemical analysis
The pH value of the sample of substrates was assayed following dilution of 1 g in
10 mL of distilled water, and by using a digital pH meter. Mycelial biomass was measured by
determination of non esterified ergosterol. A sample of 50 mg of frozen-dried sample was
used to extract non esterified ergosterol which was determined with HPLC. Pure ergosterol
(Sigma, France) was used as standard. The total phenol was determined as reported by
Lakhtar et al. (2009). Five replicates per treatment were used.
2.5.2. Elemental analysis
The instrument used for carbon and nitrogen concentrations was a Flash EA 1212
Elemental Analyzer (France). About 1 mg (0.8 - 1.2 mg) of the sample in a capsule made of
silver (assay of C and N) was burned at 920-1000°C. The quantity of each element is
expressed in percent mass. Each determination was performed in duplicate. The instrument
was calibrated with aspartic acid standard.
96