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UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

UNIVERSITÉ PAUL CÉZANNE, AIX MARSEILLE III - IMEP

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ABSTRACT<br />

Résultats et discussion. Chapitre 3: Evaluation des facteurs de croissance<br />

The aim of the present work was to optimize the conditions of solid state fermentation<br />

of Lentinula edodes on solid olive substrate (SOS) including olive twigs and leaves (OTL),<br />

olive cake (OC) where olive mill wastewater (OMW) acts as the wetting/moistening agent. To<br />

do so, the optimal formulation of substrate for L. edodes Le119 strain culture was determined.<br />

In the other hand, the growth factors (Initial pH, aeration, inoculum age, inoculum size<br />

particle size and mineral solution) were evaluated following the experimental screening<br />

design methodology using 2 5 5 1 //16 experiments. The results of a first series of experiments<br />

showed the optimal formulation of substrate for Le119 strain was OTL (20%) + OC (30%) +<br />

OMW (50%) allowing mycelial biomass production of 16,24 mg.g -1 of substrate dry weight.<br />

For a second series of experiments, the results showed that the nutriment added to the<br />

substrate exercised a negative effect on mycelial growth of L. edodes Le119 strain and total<br />

phenol removal of substrate, while the spawn size exercised a positive effect on both<br />

responses. The mycelial growth appeared to be in correlation with reduction of total phenols<br />

(r 2 = 0.81, P< 0.001). Optimized conditions of Le119 culture on SOS with 10% of spawn and<br />

without solution mineral led to production of mycelial biomass of 30.44 mg.g -1 of substrate<br />

dry weight, absence of culture contamination and to a 48% drop in total phenols initially<br />

present in OMW.<br />

Keywords: Olive twigs and leaves, Olive cake, Olive mill wastewater, Solid state<br />

fermentation, Experimental design methodology, Optimization. Phenol degradation.<br />

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