Analyysilaitteet kemian kouluopetuksessa, spektrofotometri - OuLUMA

Why is concentra:on propor:onal to absorbance? ABS = -­‐log (I/I 0 ) I 0 I 1 I 2 I 3 • So if the light goes through the first cuve;e it is reduced by 10 %, and I 1 is 90% of I 0 . The light then goes through the second cuve;e and is once again reduced 10 %, so I 2 is 81% of I 0 . Then the light goes through the third cuve;e and is reduced 10 % and the intensity of light is only 73,1 % and so on… • So when light goes through more blue colored liquid it absorbes more of the I, but in a series which can be seen as logarithmic func