TYÖN NIMI - Theseus
TYÖN NIMI - Theseus
TYÖN NIMI - Theseus
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SAVONIA UNIVERSITY OF APPLIED SCIENCES<br />
Health Professions Kuopio<br />
THESIS<br />
Abstract<br />
Degree Programme: Biomedical laboratory science<br />
Option:<br />
Authors: Haapalahti Riikka, Korhonen Helena<br />
Title of Thesis: In-House ELISA assay for microbespesific IgM antibodies, determination of reference values<br />
Date: 9.12.2009 Pages / appendices: 59/7<br />
Supervisor: Principal Lecturer Sirkka-Liisa Halimaa<br />
Contact persons: Institute of Occupational Health, Kuopio Regional Office<br />
Researcher Sirpa Pennanen, Laborant Mari Haapakoski<br />
Moisture damages are unusually common in Finland. There are many reasons for that: mistakes made during<br />
construction, false material choices and inadequate maintenance operations. Mold damages will be formed if<br />
the moisture damage is not detected and it is not repaired. Exposure to moisture and mold damages may cause<br />
health effects. Respiratory infections, nose and throat irritation, skin irritation and allergic reactions are most<br />
common symptoms and illnesses that microbes cause.<br />
The human body is exposed to billions of foreign bodies or antigens each and every day. If an antigen enters<br />
the body, the body produces antibodies against it. Antibodies bind to antigens and this prevents the antigen<br />
doing any harm. It is important to know how the body has reacted against some distraction or why it has not<br />
reacted at all. That is why immunological methods have been developed. ELISA (Enzyme-linked<br />
immunosorbent assay) is a common method in detection for human antibodies. Institute of Occupational<br />
Health, Kuopio Regional Office determine IgG and IgE antibodies with ELISA method and has started the<br />
process of validation the method for IgM antibodies.<br />
The aim of this study was to participate in the project of Institute of Occupational Health Kuopio Regional<br />
Institute to validate the method for IgM antibodies and determine reference values for IgM antibodies. The<br />
material, 200 human serum samples were collected by National Institute for Health and Welfare. People were<br />
24–64 years old living in Kuopio. In this study the IgM antibody levels for eight wet environment microbes (6panel)<br />
in 100 serum samples and eight moisture damage microbes (7 panel) in 100 serum samples were<br />
determined. The reference values were calculated so that 90 % of healthy individuals is 90 % percentile to<br />
below the threshold. The Institute of Occupational Health, Kuopio Regional Office may compare the values of<br />
patient to these reference values.<br />
Keywords: (1-5) antibody, moisture damage microbe, ELISA, reference value<br />
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