O papel modulador do gene AIRE - capes

lymphocytes and finally associates with proliferation-differentiation molecules,such as Cdk4.4) The Calpastatin gene, Cast, (mRNA accession number NM_009817, ID640722) represents a dynamic gene node that was positively self-regulated,negatively regulated the Cdk4 gene and positively regulated by four genes(Fringe, Dock8, Emb and Kif3c) and two transcribed loci (ID 640292 and ID640710). Their coded protein, calpastatin is a natural inhibitor of the enzymecalpain, which catalyzes protein cleavage.5-6) The transcribed locus (ID 640674), although anonymous, representsanother dynamic gene node, which was positively self-regulated, regulated byfive genes (Cast, Lnfg, Dock8, Emb and Kif3c) and by two anonymoustranscribed loci (ID 640292 and ID 640643). The anonymous transcribed locus(ID 640292) also featured a gene node, which was positively self-regulated,regulated by two genes (Cast and Lnfg) and by the anonymous transcribedlocus (ID 640674). It positively regulated the Dock8 gene and negativelyregulated the Cdk4 gene.7) The Dedicator of cytokinesis 8 gene, Dock8, (mRNA accession numberNM_028785, ID 639971), whose coded protein is a guanyl-nucleotidereleasing factor, featured another gene node. Of the five gene interactions,four were positive, including a feedback-like interaction with the Cast gene(Dock8 ↔ Cast) and three others; one with the Fringe gene and two with thetranscribed loci, ID 640292 and ID 640674, respectively. Moreover, Dock8negatively regulated the Cdk4 gene.The network of 14 vs 16 days p.c. is particularly interesting, due to thepresence of sequences uncharacterized in terms of their biological function,251