O papel modulador do gene AIRE - capes

gene, Prr2, (mRNA accession number NM_022999, ID 640686) and theSyntaxin binding protein 3 gene, Stxbp3, (mRNA accession numberNM_011504, ID 640484), whose respective coded proteins play a role in theinhibition of the calcium influx pathway via calcineurin, correspond to importantnodes in this and in subsequent phases.These genes participate in the control of calcium influx pathway mediated bycalcineurin, which is important in the activation of the nuclear factor of activatedT cells (Nfat), a T cell transcription factor. As calcium signaling is implicated inthe induction of T cell anergy mediated through calcineurin and NFAT [41-43],the nodes identified could be useful to provide a clearer understanding of thegene interactions implicated in the immunological tolerance induction.Twenty-one gene nodes were identified at 14 vs 15 days p.c. and eight wereselected for discussion:1) The Prr2 is a gene node featuring self positive regulation, was positivelyregulated by 19 other genes (of the 21 found in the network) and was indirectpositivelyregulated by two others, i.e. two genes interacted first and thenpositively regulated the Prr2 gene; the Cope gene positively regulated thePrmt3 gene, which positively regulated the Prr2 gene: Cope → Prmt3 → Prr2.This strongly suggests the importance of maintaining the Prr2 gene transcriptionlevels. A negative feedback-like regulation, between the Prr2 gene and GolgiSNAP receptor complex 2 gene, Gosr2, (mRNA accession numberNM_019650, ID 640152) was found. While the Gosr2 gene positively regulatesPrr2, the latter presented an inhibitory effect on Gosr2.2) The Stxbp3a gene node also featured self positive regulation and waspositively regulated by three genes participating in the network. However, this248