O papel modulador do gene AIRE - capes

Finally, to evaluate the consistency of the results obtained, the networksdeveloped by our group were compared to those reported in the literature andcompiled by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database(http://www.genome.jp/kegg/).ResultsDifferentially expressed genesTo identify significant changes in gene expression during fetal development ofthe thymus, the stage when the thymocytes mature and the onset of T cellreceptor V beta 8.1 (TRBV8.1) recombination occurs in the (♀Balb-c x♂C57Bl/6) F 1 hybrid mice (at 14 days of gestation p.c.) was compared withthose observed on subsequent days (14 vs 15, 14 vs 16 and 14 vs 17 daysp.c.). The demarcation of the emergence of TRBV8.1 recombination in thesehybrid mice was realized in a previous study by our group [7] and confirmed inthe samples used in this study (data not shown).A scatter plot of the observed relative difference d(i) versus the expectedrelative difference d E (i) was used, as shown by the SAM program.Most of the 1,576 sequences tested presented d(i)≈ d E (i), i.e., their expressionpattern did not change; however, some genes were significantly modulatedduring thymus development, which were then analyzed in the GeneNetworkprogram (Fig. 1).244