O papel modulador do gene AIRE - capes

MANUSCRITOinduction) of these genes was evaluated comparing control and Aire silenced3.10 mTECs.Reconstruction of gene networksThe GeneNetwork 1.2 algorithm [Wu et al., 2004] was used to comparemeans of different gene expression values whose standard deviation did notoverlap, whose objective was to compute a network that establishedrelationships between all genes. The linear interpolation for the networkorganization was used. The software for this algorithm can be obtained from theauthors (http://idv.sinica.edu.tw/hchuang/GeneNetwork1.2Setup.exe).In order to organize networks only with genes whose expression values weresignificant, values for the induced and repressed genes selected by the SAMstatistics were used. In this case, of the 4,500 sequences present on themicroarray, a total of 212 from control and 212 from Aire silenced 3.10 mTECsdifferentially expressed genes were included in the calculations of theGeneNetwork software due to their statistical significance.Results and DiscussionPGE exhibited by mTECs in the thymus is a complex phenomenon until nowobserved in humans and mice and involves 5-10% of all known genes of thesespecies. This process is a guarantee of self-representation of mostparenchymal tissues and organs during the central tolerance of T cells.Using RT-PCR, some of the most important autoantigens mRNAs, such asthyroglobulin, CRP, GAD67, insulin and albumin were detectable only in mTECs212