O papel modulador do gene AIRE - capes

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Anexo III
ANEXO IIITabela IV. Genes da rede controle que fazem interação com o nó gênico Gucy2d e otipo de interação.Regula<strong>do</strong>spositivamente porGucy2dRegula<strong>do</strong>snegativamente porGucy2dRegulampositivamenteGucy2dRegulamnegativamenteGucy2dGclmWdr53IMAGE:640838PrkdcCcdc41IMAGE:582951IMAGE:5828793110037I16RikIMAGE:640865Dusp2IMAGE:583053IMAGE:640664IMAGE:640521H2-K1Sfrs1IL12AOrmdl1Dnajc7Cd27Hist1h2bcArl5cIMAGE:639730IMAGE:575254IMAGE:640875Adhfe1Rbm39IMAGE:582312Gucy2dIMAGE:640272IMAGE:583031IMAGE:640280IMAGE:640860Hist1h2bcIMAGE:6403511110037N09RikTxnrd1GclmA430107D22RikE230029C05RikNol5aIMAGE:640173Rab22aMaeaCd97Ifngr2Cnot10Anxa4Hel308FosMis12Zmat5Cd8aIMAGE:583908Sfrs1IMAGE:639730Il16IMAGE:575844Sh3gl1Cdca8GzmaIMAGE:575254IMAGE:640851Adhfe1Rbm39Stx18Ifit23110031B13RikIL12AIMAGE:576142IMAGE:573897E2f2Rpl27aIMAGE:640451Mmp14Wdr53IMAGE:640838PrkdcIMAGE:639827Ccdc41IMAGE:5829513110037I16RikIMAGE:640865Dusp2Jmjd3IMAGE:6408372900076A07RikIMAGE:583456IMAGE:640667Atg2bIMAGE:640341IMAGE:582983Plekha1IMAGE:640598H13IMAGE:640121Syngr2Bcap31IMAGE:583312Cd28Zhx1FASLGFASLGCCR7FCRH3AI662270LOC729230IMAGE:639684IMAGE:641087IMAGE:1362232IMAGE:640027NlkIMAGE:640717196
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Universidade de São PauloFaculdade
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Macedo, ClaudiaO papel modulador do
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Dedico Especialmente este TrabalhoM
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AgradecimentosAgradeço do fundo do
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ÍndiceRESUMO .....................
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INDICE DE FIGURASFigura 1. Desenvol
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Promiscuous gene expression in medu
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INTRODUÇÃO1. INTRODUÇÃO1.1. A m
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INTRODUÇÃORecentemente surgiram e
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INTRODUÇÃOmigram para a região s
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INTRODUÇÃOCD4 + CD8 + , e Ccl21 (
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INTRODUÇÃOos timócitos duplo-pos
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INTRODUÇÃOgrande diversidade nas
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INTRODUÇÃOAs células mTECs são
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INTRODUÇÃODerbinski (2004). Os ti
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INTRODUÇÃOinvertebrados multicelu
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INTRODUÇÃOCoraçãoLínguaEstôma
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INTRODUÇÃO1.3. O papel do gene Au
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INTRODUÇÃOdefinido pela atividade
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INTRODUÇÃOpara abordarmos nossa p
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INTRODUÇÃOesses modelos levam em
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Hipóteses
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Objetivos
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Material e Métodos
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MATERIAL E MÉTODOSFigura 6. Padrã
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MATERIAL E MÉTODOScom estes RNAs f
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MATERIAL E MÉTODOSdos complexos de
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MATERIAL E MÉTODOSAo RNA total rec
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4.5. PCRs semi-quantitativas para o
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MATERIAL E MÉTODOSminutos a 4 °C.
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MATERIAL E MÉTODOS(Merck), soluç
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MATERIAL E MÉTODOSEnsaios de co-hi
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MATERIAL E MÉTODOSRNA TotalANELAME
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MATERIAL E MÉTODOSa) Preparação
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MATERIAL E MÉTODOScentrifugação
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MATERIAL E MÉTODOScanais (Cy3 e Cy
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MATERIAL E MÉTODOSSpotfinderQuanti
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MATERIAL E MÉTODOSOnde Xp(i) e Xc(
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MATERIAL E MÉTODOSO programa SAM p
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MATERIAL E MÉTODOSextrair automati
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DELINEAMENTO EXPERIMENTAL5. DELINEA
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RESULTADOS6. RESULTADOS6.1. Inibiç
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RESULTADOSPCR semiquantitativa gene
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RESULTADOS6.3. RT-PCRs semi-quantit
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RESULTADOS11.00%6,50%AdequadaNão A
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RESULTADOS6.6. Quantificação e no
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RESULTADOSPara um melhor entendimen
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RESULTADOSFigura 25. Agrupamento hi
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RESULTADOSFigura 26. Gráfico ilust
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RESULTADOS6.8. Influência do silen
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Discussão
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DISCUSSÃOA tolerância do repertó
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DISCUSSÃOCom relação à técnica
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DISCUSSÃOgene AIRE (do inglês aut
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DISCUSSÃOdesse modo a tolerância
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DISCUSSÃOPML (leucemia promielocit
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DISCUSSÃOBoehm et al. (2003) afirm
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DISCUSSÃOprograma Genenetwork (Wu
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Conclusões
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Referências Bibliográficas
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REFERÊNCIAS BIBLIOGRÁFICASBarthlo
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REFERÊNCIAS BIBLIOGRÁFICASDissert
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REFERÊNCIAS BIBLIOGRÁFICASGotter
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REFERÊNCIAS BIBLIOGRÁFICASMatsumo
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REFERÊNCIAS BIBLIOGRÁFICASby mito
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REFERÊNCIAS BIBLIOGRÁFICASRossi S
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REFERÊNCIAS BIBLIOGRÁFICASof thym
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Anexo I123
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ANEXO IEST IMAGE:582370 Expressed s
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ANEXO IDhps IMAGE:583332 Deoxyhypus
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ANEXO IMapkapk2 IMAGE:572979 MAP ki
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ANEXO IGm608 IMAGE:583350 Gene mode
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ANEXO ICcdc72 IMAGE:640628 Coiled-c
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ANEXO ITranscribed locusTranscribed
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ANEXO IEST IMAGE:583221 Expressed s
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ANEXO ITranscribed locusTranscribed
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ANEXO Irepair deficiency, complemen
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ANEXO Imember 1EST IMAGE:583824 Exp
Page 160 and 161: ANEXO ITranscribed locus IMAGE:5830
Page 162 and 163: ANEXO ITfdp1 IMAGE:640119 Transcrib
Page 164 and 165: ANEXO IEST IMAGE:583902 Expressed s
Page 166 and 167: ANEXO I2410022M11Rik IMAGE:640727 R
Page 168 and 169: ANEXO Icontaining 1Kif14 IMAGE:5836
Page 170 and 171: ANEXO Imember 3In multiple clusters
Page 174 and 175: ANEXO IAnkrd10 IMAGE:583740 Ankyrin
Page 178 and 179: ANEXO IVcam1 IMAGE:576563 Vascular
Page 180 and 181: ANEXO ITegt IMAGE:639877 Testis enh
Page 182 and 183: ANEXO I3Ehmt2Euchromatic histone ly
Page 188 and 189: ANEXO I20 homolog (yeast)Ube1c IMAG
Page 190 and 191: ANEXO IRasa3 IMAGE:582174 RAS p21 p
Page 194 and 195: ANEXO I4930566A11Rik IMAGE:583433 R
Page 200 and 201: Anexo II
Page 202 and 203: ANEXO II1500002B03RikIMAGE:1330385
Page 204 and 205: ANEXO IIEST IMAGE:640173 Expressed
Page 206 and 207: ANEXO IICnot10 IMAGE:576406 CCR4-NO
Page 208 and 209: ANEXO IIEST IMAGE:576142 Expressed
Page 212 and 213: ANEXO III197Ifit1RelaIMAGE:575632Gr
Page 214 and 215: ANEXO III199Nde1Nol5aIMAGE:640341Rp
Page 216 and 217: Manuscrito da Tese
Page 218 and 219: MANUSCRITOAbstractThe expression of
Page 220 and 221: MANUSCRITOdominated the scenario in
Page 222 and 223: MANUSCRITOserum autoantibodies, all
Page 224 and 225: MANUSCRITOwere transfected with 10
Page 226 and 227: MANUSCRITOwere analyzed using the M
Page 228 and 229: MANUSCRITOpurified from ex vivo mou
Page 230 and 231: MANUSCRITOinformation theory as ARA
Page 232 and 233: MANUSCRITOKyewski B, Derbinski J, G
Page 234 and 235: MANUSCRITOBruno R, Sabater L, Tolos
Page 236 and 237: MANUSCRITOFigure 3. The gene expres
Page 238 and 239: MANUSCRITOABFigure 5. Genetics netw
Page 240 and 241: SÚMULACURRICULUM VITAE (Janeiro 20
Page 242 and 243: ESTÁGIO NO EXTERIORSÚMULA• Labo
Page 244 and 245: SÚMULAtranscript finishing initiat
Page 246 and 247: SÚMULAlinhagens isogênicas de cam
Page 248 and 249: SÚMULABOLSAS DE ESTUDO• Bolsa de
Page 250 and 251: T cell signaling gene networks duri
Page 252 and 253: IntroductionThe thymus is an import
Page 254 and 255: ontogeny using such technology has
Page 256 and 257: laboratory using a Generation III A
Page 258 and 259: cDNA microarray data analysisStatis
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Gene networksAs previously mentione
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cells, including human herpesvirus-
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gene was mostly indirectly positive
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lymphocytes and finally associates
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4) Finally, Prrg2 was also identifi
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14. Kishimoto H, Sprent J (2000) Th
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proliferation of a rat astrocyte ce
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IMMUNOLOGYORIGINAL ARTICLEOnset of
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Promiscuous gene expression in muri
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R.S. Cardoso et al. / Molecular Imm
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Molecular Immunology 42 (2005) 1043
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D.A.R. Magalhães et al. / Molecula
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D.A.R. Magalhães et al. / Molecula
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Molecular and Cellular Biochemistry
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225Table 1. Target cDNA sequences a
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227coincided (CBA/J) with expressio
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