CUBETA DE ELECTROFORESIS VERTICAL VERTICAL ... - Auxilab

6. TROUBLESHOOTINGPROBLEMThe glass plates crackThe gel dye front is not straightBands are distortedLanes are broader at the bottomthan the topDye migration is slower than expectedCOMMENTSENGLISH- The wedge to hold the gel is too tight.- The voltage or current setting may be toohigh, causing overheating.- Electrophoresis buffer may be too concentrated,leading to a too high current and overheatingat a constant voltage.- Verify that the cell is level during electrophoresis- The gel may be overheated. Reduce the voltageor current. Use chilled electrophoresis buffer.- Samples may contain a excess of salt. Dialyzeor desalt before loading on gel.- Samples may be too concentrated. Dilute samplesor reduce voltage.- Samples may contain precipitate material.Centrifuge or filter before loading on gel.- The upper surface of the resolving gel maynot be flat. Use water-saturated n-butanol tooverlay the gel solution, rather than water or buffer.- Sample wells may contain small fragments ofpolyacrylamide. Rinse wells thoroughly beforeloading samples.- This is normal if adjoining lanes are not loadedwith similar samples. It is also normal forpolyacrylamyde gradient gels.- The electrophoresis buffer may be too concentrated.Check buffer preparation procedure. If theconcentration is high, at constant current the voltagewill be lower than usual.- Check voltage or current settings on powersupply.- Check for secure connections of lid and powercords.INDICE DE CONTENIDOS1. APLICACIONES DEL INSTRUMENTO2. DESCRIPCIÓN Y COMPONENTES3. ESPECIFICACIONES TÉCNICAS4. MODO DE USO5. FÓRMULAS PARA LA PREPARACIÓN DE GELES Y TAMPONES6. RESOLUCIÓN DE PROBLEMAS7. MANTENIMIENTO Y LIMPIEZAANEXO I: CERTIFICADO CE1. APLICACIONES DEL INSTRUMENTOCASTELLANOLa cubeta de electroforesis vertical de Nahita está diseñada para la separación de proteínasy ácidos nucleicos en geles de poliacrilamida. El equipo viene completo con todoslos accesorios necesarios para la preparación y electroforesis de geles de 82x82 mm.2. DESCRIPCIÓN Y COMPONENTES1. Tanque externo2. Conectores tipo banana3. Soporte para geles4. Cuña5. Tapa6. Cables fijos52623335511121315Dye migration is slower than expected- The electrophoresis buffer may be too diluted.Check buffer preparation procedure.- Check voltage or current settings on powersupply.14Page 26 Instruction manual 53001001 Version 1 May-07Revisión 1, Mayo-07 Manual de instrucciones 53001001 Pág. 3