(Eh) y metanólico (Em) de Pera distichophylla sobre un aislado de ...
(Eh) y metanólico (Em) de Pera distichophylla sobre un aislado de ... (Eh) y metanólico (Em) de Pera distichophylla sobre un aislado de ...
A. MORALES et al. Seroprevalence, Risk Factors and Comparison of Different Diagnostic Methods in Jordan. Transbound Emerg Dis. 2. ADASZEK L, GÓRNA M, KRZYSIAK M, ADAS- ZEK M, GARBAL M, WINIARCZYK S. 2011. Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland. Wiad Parazytol.; 57(1): 21-26. 3. ALUJA A, CONSTANTINO C. 2002. Technical of Necropsy in domestic animals. 2nd ed., pp 103. Manual Moderno. México. 4. BANKS W. 1996. Veterinary Applied Histology. 2nd ed., 487-492. Manual Moderno México. 5. BOTANA L, LANDONI F, MARTÍN T. 2002. Veterinary Pharmacology and therapeutical. 1 ed., pp 3-690. Madrid España. 6. BRüNING A. 1996. Equine piroplasmosis an update on diagnosis, treatment and prevention. Br Vet J. Mar; 152(2): 139-151. 7. BUTLER CM, NIJHOF AM, VAN DER KOLK JH, DE HASETH OB, TAOUFIK A, JONGEJAN F, HOUWERS DJ. 2008. Repeated high dose imidocarb dipropionate treatment did not eliminate Babesia caballi from naturally infected horses as determined by PCR-reverse line blot hybridization. Vet Parasitol. Feb 14; 151 (2-4): 320-322. 8. DE VERA M, GUILLÉN A, GARCÍA F, CONTRERAS R, SIERRALTA A, LEÓN E. 2006. Seroprevalencia de la babesiosis equina en caballos purasangre de carrera alojados en los Hipódromos La Rinconada y Nacional de Valencia, Venezuela. Veterinaria Trop. 31(1-2): 43- 52. 82 9. DONALD M. 1996. Special Veterinary Pathology. 3rd ed., 24-29. Mosby. USA. 10. FRIEDHOFF KT, TENTER AM, MüLLER I. Haemoparasites of equines: impact on international trade of horses. Rev Sci Tech. 1990 Dec; 9(4): 1187- 1194. 11. HAILAT NQ, LAFI SQ, AL-DARRAJI AM, AL-ANI FK. 1997. Equine babesiosis associated with strenuous exercise: clinical and pathological studies in Jordan. Vet Parasitol. Apr; 69(1-2): 1-8. 12. JUBB K, KENNEDY P, PALMER N. 1984. Domestic animal pathology. 3 ed., vol. 2., 59-90. Hemisferio Sur, S.R.L. Uruguay. 13. KATZ J, DEWALD R, NICHOLSON J. 2000. Procedurally similar competitive immunoassay systems for the serodiagnosis of Babesia equi, Babesia caballi, and Trypanosoma equidperdum, and Burkholderia mallei infection in horses. J Vet. Diagn. Invest 12: 46-50. 14. USDA. Seroprevalence of Equine Piroplasmosis Disease Agents in the United States. 2009. APHIS. Info Sheet. Veterinary Services. Centers for Epidemiology and Animal Health. USDA. 15. MEYER C, GUTHRIE AJ, STEVENS KB. 2005. Clinical and clinicopathological changes in 6 healthy ponies following intramuscular administration of multiple doses of imidocarb dipropionate. J S Afr Vet Assoc. Mar; 76(1): 26-32. 16. MUJICA FF, PERRONE T, FORLANO M, CORONA- DO A, MELÉNDEZ RD, BARRIOS N, ALVAREZ R, GRANDA F. 2011. Serological prevalence of Babesia caballi and Theileria equi in horses of Lara State, Venezuela. Vet Parasitol. May 31; 178(1-2): 180-183. Rev. Ibero-Latinoam. Parasitol. (2012); 71 (1): 78-82
Artículo Original Rev. Ibero-Latinoam. Parasitol. (2012); 71 (1): 83-89 Detección de ooquistes de Cryptosporidium spp. en la planta de tratamiento de aguas residuales “Taiguaiguay” del Estado Aragua, Venezuela año 2011 MEDINA C. 1 , MONCADA E. 1 , GONZALEZ A. 1 , RUEDA M. 2 , y ROJAS G. 1 1 Universidad de Carabobo, Facultad de ciencias de la Salud, Escuela de Bioanálisis. 2 Universidad Central de Venezuela, Facultad de ciencias Veterinarias, Escuela de Veterinaria. ABSTRACT DETECTION OF CRYPTOSPORIDIUM OOCYSTS IN THE WATER TREATMENT RESIDUAL PLANT OF TAIGUAIGUAY, ESTADO ARAGUA, VENEzUELA Cryptosporidiosis is a gastrointestinal parasitic disease of man and animals caused by a opportunistic protozoan called Cryptosporidium. It is a zoonosis that can be acquired through human and animal feces. The infective stage is the oocyst, which is resistant to adverse conditions in the environment. In Latin America there is an increase of mortality due to high prevalence in patients infected with Human Immunodeficiency Virus (HIV). In the present investigation was assessed by The presence of Cryptosporidium in the water treatment plant residual Taiguaiguay Venezuelan Aragua State was assessed by direct immunofluorescence (DIF). We analyzed 14 samples of water (7 at the entrance and 7 in the output of the water treatment plant) resulting 57% of the samples collected at the entrance of the plant contaminated with Cryptosporidium oocysts and 43% at the start, with an overall average of 50% of contaminated water with oocysts (maximum and minimum values found in the input were 16 Ooq / L and 4 Ooq / L at the output of 6Ooq / L and 3 Ooq / L), representing a risk level for human consumption. Key words: Wastewater, Cryptosporidium spp, direct immunofluorescence (DIF). RESUMEN La criptosporidiosis es una enfermedad parasitaria gastrointestinal del hombre y de los animales causada por un protozoario patógeno denominado Cryptosporidium. Es una zoonosis que puede adquirirse a través de heces de animales y humanos, El ooquiste es el estadio infectivo, resistente a condiciones adversas en el medio ambiente. En Latinoamérica existe un incremento de morbimortalidad debido a una alta prevalencia en pacientes infectados por el Virus de Inmunodeficiencia Humana (VIH). En la presente trabajo se evaluó Recibido: 18 de Febrero de 2012. Aceptado: 30 de Mayo de 2012. Correspondencia: Claudio Medina E-mail: set504@hotmail.com / set504@yahoo.com 0412-8310593. 83
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A. MORALES et al.<br />
Seroprevalence, Risk Factors and Comparison of Different<br />
Diagnostic Methods in Jordan. Transbo<strong>un</strong>d <strong>Em</strong>erg<br />
Dis.<br />
2. ADASZEK L, GÓRNA M, KRZYSIAK M, ADAS-<br />
ZEK M, GARBAL M, WINIARCZYK S. 2011. I<strong>de</strong>ntification<br />
of the piroplasms isolated from horses with clinical<br />
piroplasmosis in Poland. Wiad Parazytol.; 57(1):<br />
21-26.<br />
3. ALUJA A, CONSTANTINO C. 2002. Technical of<br />
Necropsy in domestic animals. 2nd ed., pp 103. Manual<br />
Mo<strong>de</strong>rno. México.<br />
4. BANKS W. 1996. Veterinary Applied Histology. 2nd<br />
ed., 487-492. Manual Mo<strong>de</strong>rno México.<br />
5. BOTANA L, LANDONI F, MARTÍN T. 2002. Veterinary<br />
Pharmacology and therapeutical. 1 ed., pp 3-690.<br />
Madrid España.<br />
6. BRüNING A. 1996. Equine piroplasmosis an update<br />
on diagnosis, treatment and prevention. Br Vet J. Mar;<br />
152(2): 139-151.<br />
7. BUTLER CM, NIJHOF AM, VAN DER KOLK JH,<br />
DE HASETH OB, TAOUFIK A, JONGEJAN F,<br />
HOUWERS DJ. 2008. Repeated high dose imidocarb<br />
dipropionate treatment did not eliminate Babesia<br />
caballi from naturally infected horses as <strong>de</strong>termined by<br />
PCR-reverse line blot hybridization. Vet Parasitol. Feb<br />
14; 151 (2-4): 320-322.<br />
8. DE VERA M, GUILLÉN A, GARCÍA F, CONTRERAS<br />
R, SIERRALTA A, LEÓN E. 2006. Seroprevalencia <strong>de</strong><br />
la babesiosis equina en caballos purasangre <strong>de</strong> carrera<br />
alojados en los Hipódromos La Rinconada y Nacional<br />
<strong>de</strong> Valencia, Venezuela. Veterinaria Trop. 31(1-2): 43-<br />
52.<br />
82<br />
9. DONALD M. 1996. Special Veterinary Pathology. 3rd<br />
ed., 24-29. Mosby. USA.<br />
10. FRIEDHOFF KT, TENTER AM, MüLLER I.<br />
Haemoparasites of equines: impact on international<br />
tra<strong>de</strong> of horses. Rev Sci Tech. 1990 Dec; 9(4): 1187-<br />
1194.<br />
11. HAILAT NQ, LAFI SQ, AL-DARRAJI AM, AL-ANI<br />
FK. 1997. Equine babesiosis associated with strenuous<br />
exercise: clinical and pathological studies in Jordan.<br />
Vet Parasitol. Apr; 69(1-2): 1-8.<br />
12. JUBB K, KENNEDY P, PALMER N. 1984. Domestic<br />
animal pathology. 3 ed., vol. 2., 59-90. Hemisferio Sur,<br />
S.R.L. Uruguay.<br />
13. KATZ J, DEWALD R, NICHOLSON J. 2000. Procedurally<br />
similar competitive imm<strong>un</strong>oassay systems for<br />
the serodiagnosis of Babesia equi, Babesia caballi, and<br />
Trypanosoma equidperdum, and Burkhol<strong>de</strong>ria mallei<br />
infection in horses. J Vet. Diagn. Invest 12: 46-50.<br />
14. USDA. Seroprevalence of Equine Piroplasmosis Disease<br />
Agents in the United States. 2009. APHIS. Info<br />
Sheet. Veterinary Services. Centers for Epi<strong>de</strong>miology<br />
and Animal Health. USDA.<br />
15. MEYER C, GUTHRIE AJ, STEVENS KB. 2005.<br />
Clinical and clinicopathological changes in 6 healthy<br />
ponies following intramuscular administration of<br />
multiple doses of imidocarb dipropionate. J S Afr Vet<br />
Assoc. Mar; 76(1): 26-32.<br />
16. MUJICA FF, PERRONE T, FORLANO M, CORONA-<br />
DO A, MELÉNDEZ RD, BARRIOS N, ALVAREZ R,<br />
GRANDA F. 2011. Serological prevalence of Babesia<br />
caballi and Theileria equi in horses of Lara State, Venezuela.<br />
Vet Parasitol. May 31; 178(1-2): 180-183.<br />
Rev. Ibero-Latinoam. Parasitol. (2012); 71 (1): 78-82