[SAS-MX Acid Hb]. - AgentÃºra Harmony vos

More documents

Recommendations

Info

$Adaptaciones Cobas Mira $EspaÃ±ol$ - AgentÃºra Harmony vos$

INTERPRETATION OF RESULTSQualitative Evaluation:The possible identity of the haemoglobin types present in the samples can be determined by visualevaluation of the completed gel. The Hemo controls provide a marker for band identification.Figure 1 shows the possible identity of the most commonly encountered haemoglobin types.F AA 2DEGJHO S CMost haemoglobin variants cause no discernible clinical symptoms, so are of interest primarily toresearch scientists. Variants are clinically important when their presence leads to sickling disorders,thalassaemia syndromes, life long cyanosis, haemolytic anaemias or erythrocytosis, or if theheterozygote is of sufficient prevalence to warrant genetic counselling. The combinations of HbS-S,HbS-D-Los Angeles, and HbS-O Arab lead to serious sickling disorders 2 . Several variants includingHbH, E-Fort Worth and Lepore cause a thalassaemic blood picture 2 .The two variant hemoglobins of greatest importance in terms of frequency and pathology are HbS andHbC 2 . Sickle cell anaemia (HbSS) is a cruel and lethal disease. It first manifests itself at about 5-6months of age. The clinical course presents agonising episodes of pain and temperature elevations withanaemia, listlessness, lethargy, and infarct in virtually all organs of the body.The individual with homozygous HbCC suffers mild haemolytic anaemia which is attributed to theprecipitation or crystallization of HbC within the erythrocytes. Cases of HbSC disease arecharacterised by haemolytic anaemia that is milder than sickle-cell anaemia.The thalassaemias are a group of haemoglobin disorders characterised by hypochromia andmicrocytosis due to the diminished synthesis of one globin chain (the α or β) while synthesis of theother chain proceeds normally 9,10 . This unbalanced synthesis results in unstable globin chains. Theseprecipitate within the red cell, forming inclusion bodies that shorten the life span of the cell.In α-thalassaemias, the α chains are diminished or absent, and in the β-thalassaemia, the β chains areaffected. Another quantitative disorder of haemoglobin synthesis, hereditary persistent foetalhaemoglobin (HPFH), represents a genetic failure of the mechanisms that turn off gamma chainsynthesis at about four months after birth, which results in a continued high percentage of HbF. It is amore benign condition than the true thalassaemias and persons homozygous for HPFH have normaldevelopment, are asymptomatic and have no anemia 10 .4
SAS-MX ACID HB-10The most common haemoglobin abnormalities:Sickle Cell TraitThis is a heterozygous state showing HbA and HbS and a normal amount of HbA 2 on cellulose acetate.Results on citrate agar show haemoglobins in the HbA and HbS migratory positions (zones).Sickle Cell AnaemiaThis is a homozygous state showing almost exclusively HbS, although a small amount of HbF may alsobe present.Sickle-C DiseaseThis is a heterozygous state demonstrating HbS and HbC.Sickle CeIl-Thalassaemia DiseaseThis condition shows HbA, HbF, HbS, and HbA 2 .In Sickle Cell b o -Thalassaemia HbA is absent.In Sickle Cell b + -Thalassaemia HbA is present in reduced quantities.Thalassaemia-C DiseaseThis condition shows HbA, HbF and HbC.C DiseaseThis is a homozygous state showing almost exclusively HbCThalassaemia MajorThis condition shows HbF, HbA and HbA 2 .LIMITATIONSSome abnormal hemoglobins have similar electrophorotic mohilities and must be differentiated byother methodologies.Further testing required:1. Globin chain analysis (both acid and alkaline) and structural studies may be necessary in order topositively identify some of the more rare haemoglobins.2. Anion exchange column chromatography is the most accurate method for quantitating HbA 2 .Helena BioSciences Sickle-Thal Quik Column Method (Cat. No. 5334) for quantitation of HbA 2 inthe presence of HbS, or the Helena BioSciences Beta-Thal HbA 2 Quik Column Procedure(Cat. No. 5341) are recommended. HbA 2 quantitation is one of the most important diagnostictests in the diagnosis of B-thalassaemia trait.3. Low levels of HbF (1-10%) may be accurately quantitated by radial immunodiffusion using theHelena BioSciences HbF-QuiPlate Procedure (Cat. No. 9325).REFERENCE VALUESAt birth, the majority of haemoglobin in the erythrocytes of the normal individual is foetal haemoglobin,HbF. Some of the major adult haemoglobin, HbA, and a small amount of HbA 2 , are also present.At the end of the first year of life and through adulthood, the major haemoglobin present is HbA withup to 3.7% HbA 2 and less than 2% HbF.5English
Page 1: helenawww.helena-biosciences.comBio
Page 4 and 5: COMPOSITION1. SAS-MX Acid Hb GelCon
Page 8 and 9: PERFORMANCE CHARACTERISTICSa) Repro
Page 10 and 11: COMPOSITION1. Plaque SAS-MX Hb acid
Page 12 and 13: 11. Décolorer le gel dans 2 bains
Page 14 and 15: PERFORMANCESa) ReproductibilitéLe
Page 16 and 17: INHALT1. SAS-MX Säure Hb GelEnthä
Page 18 and 19: 9. Das Gel 15 Minuten in die Färbe
Page 20 and 21: REFERENZWERTEBei der Geburt besteht
Page 22 and 23: 3. Colorante acido viola concentrat
Page 24 and 25: INTERPRETAZIONE DEI RISULTATIValuta
Page 26 and 27: CARATTERISTICHE PRESTAZIONALIa) Rip
Page 28 and 29: COMPOSICIÓN1. Gel Hb Ácida SAS-MX
Page 30 and 31: 10. Aclarar el gel brevemente en so
Page 32: VALORES DE REFERENCIAAl nacer, la m

[SAS-MX Acid Hb]. - AgentÃºra Harmony vos

Create successful ePaper yourself

Delete template?

Save as template?