B. bacilliformis - BVS - INS

AFLP analysis
• Standard Europena Working Group on Legionella
Infection protocol (Fry, N. K., J. M. Bangsborg, S. Bernander, J.
Etienne, B. Forsblom, V. Gaia, P. Hasenberger, D. Lindsay, A. Papoutsi,
C. Pelaz, M. Struelens, S. A.Uldum, P. Visca, and T. G. Harrison. 2000.
Assessment of intercentre reproducibility and epidemiological
concordance of Legionella pneumophila serogroup 1 genotyping by
amplified fragment length polymorphism analysis. Eur. J. Clin.
Microbiol. Infect. Dis. 19:773–780).
- Be used selective primer PstI-C
- Adapter-oligonucleotide (AFLP-LG1, 5-
CTCGTAGACTGCGTACATGCA-3;
AFLP-LG2, 5-TGTACGCAGTCTAC-3).