SERION ELISAclassic - virion\serion

Infections with ECHO viruses can be confirmed by direct detection of the pathogen or
serological evidence of specific antibodies. The PCR is an important complement to the
classical methods while in recent years ELISA tests have established themselves. Due to
the large number of serotypes a test with broad reactivity is required in order to detect all
the various serotypes with one test. The SERION ELISA classic Echovirus tests are based
on a mixture of recombinant antigens derived from conserved and subtype specific
epitopes of the VP1 proteins of ECHO viruses E6 and E9. These epitopes have been
determined to be sufficiently cross-reactive to enable the detection of infections caused by
other ECHO virus serotypes.
EN
An acute ECHO viruses infection can be confirmed by the detection of specific IgM and /or
IgA antibodies or a rising IgG titer. The highest frequency of IgM positive sera are found in
children between the ages of one and ten years. IgM antibodies are usually detectable for
a period of six to eight weeks. In rare cases IgM titers may persist up to six months after
aseptic meningitis or three to six months following pericarditis. Patients with recurrent
pericarditis may have IgM detectable for more than five years. IgA detection in cases of
acute infection is a valuable supplement to IgM serology. Persistent IgA may be detectable
in cases of type 1 diabetes and chronic heart disease for six months up to several years.
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3 TEST PRINCIPLE SERION ELISA classic
The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is
particularly suited to the determination of antibodies in the field of infectious serology. The
reaction is based on the specific interaction of antibodies with their corresponding antigen.
The test strips of the SERION ELISA classic microtiter plate are coated with specific
antigens of the pathogen of interest. If antibodies in the patient´s serum sample are
present, they bind to the fixed antigen. A secondary antibody, which has been conjugated
with the enzyme alkaline phosphatase, detects and binds to the immune complex. The
colourless substrate p-nitrophenylphosphate is then converted into the coloured product
p-nitrophenol. The signal intensity of this reaction product is proportional to the
concentration of the analyte in the sample and is measured photometrically.
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