SERION ELISAclassic - virion\serion

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Pos: 27 /Ar bei tsanl eitungen ELISA cl assic/Gültig für nur ein D okument/Testdurchführ ung/T establauf/Echovir us: Testabl auf @ 6\mod_1256123753519_18.doc @ 22974 @ Pos: 28 /Ar bei tsanl eitungen ELISA cl assic/Gültig für all e D okumente/ELISA classic/T estdurchführung/Testabl auf/T establ auf @ 4\mod_1255347017982_18.doc @ 21336 @ Pos: 29 /Ar bei tsanl eitungen ELISA cl assic/Gültig für mehrer e D okumente/T estdurchführ ung/M anuelle Testdurchführ ung (für ALLE Erreg er auß er C oxiella) @ 5\mod_1255349441824_18.doc @ 21511 @ 2 7.4 Overview - Test Procedure EN <strong>SERION</strong> ELISA classic Echovirus IgA/IgG/IgM quantitative In case of IgM detection absorption of rheumatoid factor, see No. 7.2.1; Incubation 15 minutes at room temperature or over night at 4°C sample dilution 1 (patient´s samples) IgA/IgM: 1 + 100 IgG: 1 + 500 Pipette diluted samples and ready-to-use control / standard sera into the microtest wells (100 µl) INCUBATION 60 Min./ 37 °C moist chamber WASH (4 x 300 µl DIL WASH )² Pipette conjugate solution APC (100 µl) INCUBATION 30 Min./ 37 °C moist chamber WASH (4 x 300 µl DIL WASH )² Pipette substrate solution pNPP (100 µl) INCUBATION 30 Min./ 37 °C moist chamber Pipette stopping solution STOP (100 µl) READ EXTINCTION at 405 nm 1 Special dilution buffers for the following <strong>SERION</strong> ELISA classic tests: Borrelia burgdorferi IgG, IgM, EBV EA IgG, Parvovirus B19 IgM and Hantavirus Puumala IgG, IgM 2 For manual use: tap plate at the end of the wash procedure on paper towel. english 11
Pos: 30 /Ar bei tsanl eitungen ELISA cl assic/Gültig für all e D okumente/ELISA classic/T estdurchführung/Automatische Testdurchführung @ 5\mod_1255350306938_18.doc @ 21543 @ 2 7.5 Manual Test Procedure 1. Place the required number of cavities in the frame and prepare a protocol sheet. 2. Add each 100 µl of diluted sample or ready-to-use controls into the appropriate wells of microtiter test strips. Spare one well for substrate blank, e.g.: IgA/IgG/IgM quantitative well no. well A1 well B1 well C1 well D1 Substrate blank Negative Control Standard serum Standard serum well E1 Patient 1.... 3. Sample incubation for 60 minutes (+/- 5 min) at 37 °C (+/- 1°C) in moist chamber 4. After incubation wash all wells with washing solution (by automated washer or manually): - aspirate or shake out the incubation solution - fill each well with 300 µl washing solution - aspirate or shake out the washing buffer - repeat the washing procedure 3 times (altogether 4 times!) - dry by tapping the microtiter plate on a paper towel 5. Addition of conjugate Add 100 µl of the ready-to-use IgA/IgG/IgM conjugate to the appropriate wells (except substrate blank) 6. Conjugate incubation for 30 minutes (+/- 1 min)* at 37 °C (+/- 1 °C) in moist chamber. 7. After incubation wash all wells with washing solution (see above) 8. Addition of substrate Add 100 µl of ready-to-use substrate solution to each well (including well for substrate blank!) 9. Substrate incubation for 30 minutes (+/- 1 min)* at 37 °C (+/- 1 °C) in moist chamber. 10. Stopping of the reaction Add 100 µl stopping solution to each well, shake microtiter plate gently to mix. 11. Read extinction Read optical desity (OD) within 60 minutes at 405 nm against substrate blank, reference wave length between 620 nm and 690 nm (e.g. 650 nm). _____________________ * Please note, that under special working-conditions internal laboratory adaptations of the incubation times may be necessary. english 12
Page 1 and 2: Manufacturer Fabricante Κατασ
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Page 5 and 6: 4 KIT COMPONENTS Test Components Pi
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Page 23 and 24: === Ende der Liste für T extmar ke
Page 27 and 28: 4 COMPONENTES DEL KIT Componentes d
Page 35 and 36: 7.5 Procedimiento manual 1. Coloque
Page 43 and 44: 9.2 Reproducibilidad Pos: 50 /Ar be
Page 45 and 46: === Ende der Liste für T extmar ke
Page 49 and 50: 4 ΠΕΡΙΕΧΟΜΕΝΟ ΚΑΙ Σ
Page 57 and 58: 7.5 Χειροκίνητη διαδ
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Pos: 44 /Ar bei tsanl eitungen ELIS
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9.2 Επαναληψιµότητα
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=== Ende der Liste für T extmar ke
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Pos: 1 /Arbeitsanl eitung en ELISA
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4 COMPONENTES DO KIT Componentes do
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7.5 Realização do teste pelo mét
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9.2 Reprodutibilidade Pos: 50 /Ar b
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Pos: 1 /Arbeitsanl eitung en ELISA
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4 SLOŽENÍ KITU Složky testu Odd
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7.5 Postup při ručním zpracován
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AK CAG STD POS C/O NEG APC Antikör
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SERION ELISA classic 102 Masern Vir
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SERION ELISAclassic - virion\serion

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