Applications/Columns HPLC - Mrclab.com
Applications/Columns HPLC - Mrclab.com
Applications/Columns HPLC - Mrclab.com
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<strong>HPLC</strong> Application<br />
<br />
Food<br />
<br />
No.03276<br />
<br />
No.03271<br />
1.Citrus Red 2<br />
1.Bezoic Acid<br />
2.Sorbic acid<br />
1<br />
2<br />
1<br />
1<br />
0.0<br />
2.5 5.0 7.5<br />
Min<br />
0 5 10<br />
Min<br />
15<br />
<br />
Column : Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Acetonitrile/water(80/20)<br />
Flow Rate: 1.0 mL/min<br />
Det: 500 nm<br />
Temp: 35 ˚C<br />
Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: 20mM Ammonium acetate/Methanol (90/10)<br />
Flow Rate: 1.0 mL/min<br />
Det: 230 nm<br />
Temp: 25 ˚C<br />
<br />
No.03268<br />
<br />
No.03274<br />
1.New Red<br />
2.Tartrazine<br />
3.Amaranth<br />
4.Indigotine<br />
5.Ponceau 4RC<br />
6.Sunset Yellow<br />
7.Brilliant Blue FCF<br />
8.Erythrosin B disodium salt<br />
1.Sudan I<br />
2.Sudan II<br />
3.Sudan III<br />
4.Sudan IV<br />
1<br />
8<br />
1<br />
2<br />
3<br />
4<br />
5<br />
6<br />
2<br />
3<br />
4<br />
7<br />
0<br />
5 10 15<br />
Min<br />
0 5 10<br />
Min<br />
Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: A: MethanolB20mM Ammonium acetate (pH 4.0)<br />
0min B:80%5min B:65%12min B:2%<br />
Flow Rate: 1.0 mL/min<br />
Det: 254 nm<br />
Temp: 25 ˚C<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Acetonitrile /Water (95/5)<br />
Flow Rate: 1.0 mL/min<br />
Det: 500 nm<br />
Temp: 35 ˚C
No.03267<br />
<br />
No.03231<br />
1.Nicotinamide<br />
1<br />
1.Glucose<br />
2.L-xylose<br />
3.Fructose<br />
4.Lyxose<br />
3<br />
4<br />
1 2<br />
2<br />
0 5 10<br />
Min<br />
0 4 8 12 16 20<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Column:<br />
MRCSep Ca-H 7.8 300mm, 5m (8.7830C3.0001)<br />
<br />
Eluent: Methanol / 2-Propanol /5mMSodium 1-heptanesulfonate<br />
7/2/91)Perchloric acid PH 2.1<br />
Flow Rate: 1.0 mL/min<br />
Det: 261 nm<br />
Eluent:<br />
Flow Rate:<br />
Det:<br />
Temp:<br />
Water<br />
0.6 mL/min<br />
192 nm<br />
85 ˚C<br />
Temp:<br />
25 ˚C<br />
<br />
No.03253<br />
<br />
No.03257<br />
1.Thiamin HCl<br />
2.Riboflavin<br />
7<br />
8<br />
9<br />
10<br />
1<br />
11<br />
12<br />
2<br />
13<br />
14<br />
15<br />
16 1718<br />
19 20<br />
21 22 23 24 25 26<br />
0 5<br />
10 15<br />
Min<br />
20 25<br />
5 15 25 35<br />
Min<br />
Column: Athena C18 4.6 150mm, 5m (8.461571.0001)<br />
Eluent: Acetonitrile /10mM Na3PO4 (pH 5.0)=15/85<br />
Flow Rate: 1.0 mL/min<br />
Det: 254 nm<br />
Temp: 40 ˚C<br />
Column: Athena C4 4.6 150mm, 5m (8.461579.0001)<br />
Eluent: Methanol/Water2.5/97.5<br />
Flow Rate: 1.0 mL/min<br />
Det: RID<br />
Temp: 40 ˚C
<strong>HPLC</strong> Application<br />
<br />
No.03296<br />
Industrial<br />
1.-Tocopherol<br />
2.-Tocopherol<br />
3.-Tocopherol<br />
4.-Tocopherol<br />
<br />
No.03280<br />
1<br />
4<br />
1.BBP<br />
2.DBP<br />
3.DNHP<br />
4.DEHP<br />
5.DNOP<br />
6.DINP<br />
7.DIDP<br />
3<br />
2<br />
4<br />
1<br />
2 3<br />
5<br />
3<br />
6<br />
7<br />
0 10 20 30<br />
Min<br />
Column: Athena Silica 4.6 150mm, 5m (8.461576.0001)<br />
Eluent: N-hexan/ethanol 99/1<br />
Flow Rate: 0.7 mL/min<br />
Det: 280 nm<br />
Temp: 40 ˚C<br />
0 5 10 15 20 25<br />
Min<br />
Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: t(min) Acetonitrile Water Eluent (mL/min)<br />
0 90 10 1.0<br />
6.5 100 0 1.5<br />
7.5 100 0 1.5<br />
Det: 228 nm<br />
Temp: 25 ˚C<br />
<br />
<br />
No.03247<br />
1.Vitamin C<br />
2.Vitamin B13<br />
3.Nicotinic acid<br />
4.Nicotinamide<br />
5.Pyridoxal<br />
6.Pyridoxine<br />
7.Calcium pantothenate<br />
8.Thiamin<br />
Pesticide<br />
<br />
No.03260<br />
1<br />
1.Glyphosate<br />
1<br />
2<br />
3 4<br />
6<br />
5<br />
8<br />
7<br />
0 2 4 6 8 10 12<br />
Min<br />
0 2 4 6 8<br />
Min<br />
Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: Acetonitrile/20mM H3PO4+5mM 1-pentanesulfonate sodium<br />
buffer 8/92<br />
Flow Rate: 1.0 mL/min<br />
Det: 210 nm<br />
Temp: 40 ˚C<br />
Column: Athena SAX 4.6 150mm, 5m (8.461521.0001)<br />
Eluent: methanol/10mM KH2PO4(pH 2.0)(15/85)<br />
Flow Rate: 1.0 mL/min<br />
Det: 195 nm<br />
Temp: 25 ˚C
No.03277<br />
Application veterinary drugs<br />
1.Aldicarb-sulfoxide<br />
2.Aldicarb-sulfone<br />
3.Methomyl<br />
4.Carbofuran-3-hydroxy<br />
3<br />
5.Aldicarb<br />
6.Carbofuran<br />
7.Carbaryl<br />
7<br />
<br />
1.Norfloxacin<br />
2.Ciprofloxacin<br />
1<br />
No.03278<br />
1<br />
5<br />
2<br />
4<br />
6<br />
2<br />
4<br />
10 15<br />
20<br />
Min<br />
<br />
Column : Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: t(min) Water% Methnal% Flow rate ( ml/min)<br />
0 85 15 0.5<br />
2 75 25 0.5<br />
8 75 25 0.5<br />
9 60 40 0.8<br />
10 55 45 0.8<br />
19 20 80 0.8<br />
25 20 80 0.8<br />
26 85 15 0.5<br />
Det:<br />
FLDex 330nmem 465nm<br />
post-column<br />
derivation:<br />
Oven:<br />
50mM NaOH and OPAflow rate 0.3ml/minhydrolyze<br />
Temp 100Cderivation : room temp<br />
42 ˚C<br />
0 5 10<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent : 25mM –PO4 Buffer/ Acetonitrile (85/15)<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 25 ˚C<br />
<br />
No.03248<br />
<br />
No.03251<br />
1.Thiabendazole<br />
2.o-Phenylphenol<br />
3.Biphenyl<br />
2<br />
3<br />
1.Sulfanilamide<br />
2.Sulfisomidine<br />
3.Sulfadiazine<br />
4.Sulfamethazine<br />
5.Sulfamonomethoxine<br />
1<br />
1<br />
2<br />
4<br />
3<br />
5<br />
0 2 4 6 8 10 12<br />
Min<br />
0 5 15 20 25<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Acetonitrile /30mM NH4H2PO4 buffer65/35<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 40 ˚C<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Acetonitrile /10mM H3PO4 buffer15/85<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 40 ˚C
<strong>HPLC</strong> Application<br />
<br />
No.03273<br />
<br />
No.03272<br />
1.Sulfadiazine<br />
2.Sulfamethazine<br />
3.Sulfamonomethoxine<br />
4.Sulfamethoxazole<br />
5.Sulfachinoxalin<br />
1.Oxytetracycline<br />
2.Tetracycline<br />
3.Chlortetracycline<br />
1<br />
2<br />
1<br />
3<br />
2<br />
4<br />
3<br />
5<br />
0.0 2.5 5.0 7.5 10.0<br />
Min<br />
5<br />
0 5 10 15<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Acetonitrile /water/ acetic acid (25/75/0.3)<br />
Flow rate: 1.0 mL/min<br />
Det: 270 nm<br />
Oven: 25 ˚C<br />
Application environment<br />
<br />
1.o-nitroaniline<br />
2.m-nitroaniline<br />
3.p-nitroaniline<br />
2<br />
20<br />
No.03297<br />
Column : Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: Acetonitrile /methnal/10mM Oxalic acid (15/15/70)<br />
Flow rate: 1.0 mL/min<br />
Det: 355 nm<br />
Oven: 25 ˚C<br />
<br />
1.Oxamyl<br />
2.Methomyl<br />
3.Aldicarb<br />
4.Simazine<br />
5.Monuron<br />
6.Cyanazine<br />
7.Metribuzin<br />
8.Carbofuran<br />
56<br />
1 2 4<br />
7<br />
9.Atrazine<br />
10.Carbaryl<br />
10.Fluometuron<br />
11.Diuron<br />
12.Propham<br />
13.Propachlor<br />
14.Linuron<br />
9 10 11 12 13 14<br />
No.03275<br />
<br />
1<br />
3<br />
3<br />
8<br />
0 8 16<br />
24<br />
0 5 10 15 20 25<br />
Min<br />
Column: Athena Silica 4.6 150mm, 5m (8.461576.0001)<br />
Eluent: N-HEXAN / TRICHLORMETHAN40/60<br />
Flow rate: 0.7 mL/min<br />
Det: 254 nm<br />
Oven: 25 ˚C<br />
Min<br />
Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: A: Water/ Acetonitrile (90/10)BAcetonitrile<br />
0min B:20%5min B:20%30min B:70%<br />
Flow rate: 1.5 mL/min<br />
Det: 220 nm<br />
Oven: 25 ˚C
1.Naphthalene<br />
2.Acenaphthylene<br />
3.Fluorene<br />
4.Acenaphthene<br />
5.Phenanthrene<br />
6.Anthracene<br />
7.Fluoranthene<br />
8.Pyrene<br />
9.Chrysene<br />
No.03266<br />
10.Benzo(a)anthracene<br />
11.Benzo(b)fluoranthene<br />
12.Benzo(k)fluoranthene<br />
13.Benzo(a)pyrene<br />
14.Dibenzo(a,h)anthracene<br />
15.Benzo(g,h,i)perylene<br />
16.Indeno(1,2,3-cd)pyrene<br />
Application Pharmaceuticals<br />
<br />
1.Piroxicam<br />
2.Sulindac<br />
3.Ketoprofen<br />
<br />
4.Naproxen<br />
5.Flurbiprofen<br />
6.Ibuprofen<br />
No.03237<br />
6<br />
<br />
<br />
6<br />
<br />
0<br />
2<br />
1<br />
3<br />
4<br />
5<br />
Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: A: AcetonitrileBwater<br />
0min B:35%27 min B:35%41min B:0%<br />
Flow rate: 1.2 mL/min<br />
Det: 254 nm<br />
Oven: 25 ˚C<br />
7<br />
8<br />
9<br />
10<br />
12<br />
11 13<br />
15 30 45<br />
Min<br />
14<br />
15,16<br />
<br />
<br />
<br />
0 5 10 15<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Acetonitrile /1% acetic acid buffer65/35<br />
Flow rate: 1.0 mL/min<br />
Det: 260 nm<br />
Oven: 40 ˚C<br />
<br />
No.03238<br />
<br />
1.Bishphenol A<br />
No.03281<br />
1.Protriptyline<br />
2.Desipramine<br />
3.Nortriptyline<br />
4.Doxepin<br />
5.Imipramine<br />
6.Amitriptyline<br />
7.Trimipramine<br />
1<br />
2<br />
3<br />
1<br />
4<br />
6<br />
5<br />
7<br />
0 1 2 3 4 5 6 7<br />
Min<br />
Column : Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: AAcetonitrileBWater<br />
0min B:40%7min B:5%<br />
Flow rate: 1.0 mL/min<br />
Det: 216 nm<br />
Oven: 35 ˚C<br />
0<br />
15 30<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Methnal/20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 70/30<br />
Flow rate: 1.2 mL/min<br />
Det: 240 nm<br />
Oven: 30 ˚C
<strong>HPLC</strong> Application<br />
<br />
No.03249<br />
<br />
No.03243<br />
1. Estriol<br />
2.-Estradiol<br />
3.Estrone<br />
4.Progesterone<br />
1.Cinchonine<br />
2.Cinchonidine<br />
3.Quinidine<br />
4.Quinine<br />
1<br />
2<br />
3<br />
4<br />
1 2<br />
3 4<br />
7<br />
0 4 8 12 16 20<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent:<br />
Flow rate:<br />
Det:<br />
Oven:<br />
Acetonitrile /Water60/40<br />
1.0 mL/min<br />
30 nm<br />
35 ˚C<br />
0 6 12 18<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent:<br />
Flow rate:<br />
Det:<br />
Oven:<br />
Methanol/20mM KH2PO4 buffer35/65<br />
1.0 mL/min<br />
235 nm<br />
40 ˚C<br />
<br />
No.03240<br />
<br />
No.03246<br />
1.Pheniramine<br />
2.Doxylamine<br />
3.Chlorpheniramine<br />
4.Diphenhydramine<br />
1.Primidone<br />
2.Phenytoin<br />
3.Carbamazepine<br />
3<br />
2<br />
4<br />
1<br />
2<br />
1<br />
3<br />
0 6 12 18 24 30<br />
Min<br />
0 6<br />
Min<br />
12 18<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Methanol/20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 60/40<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 40 ˚C<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Acetonitrile /20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 70/30<br />
Flow rate: 1.0 mL/min<br />
Det: 220 nm<br />
Oven: 30 ˚C
No.03241 No.03242<br />
1.Atenolol<br />
2.Nadolol<br />
3.Metoprolol<br />
4.Propranolol<br />
5.Alprenolol<br />
1.Uracil<br />
2.Procainamide<br />
3.N-Acetylprocainamide<br />
1<br />
3<br />
2<br />
3<br />
1<br />
2<br />
4<br />
5<br />
8<br />
<br />
0 5 10<br />
Min<br />
Column : Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Methanol/20mM KH2PO4 - K2HPO4 buffer(pH 7.0)60/40<br />
Flow rate: 1.0 mL/min<br />
Det: 220 nm<br />
Oven: 40 ˚C<br />
0 2 4 6 8 10<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Methanol/20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 40/60<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 40 ˚C<br />
<br />
No.03245<br />
<br />
No.03244<br />
1.Acetaminophen<br />
2.Caffeine<br />
3.Chlorpheniramine Maleate<br />
4.Aspirine<br />
5.Ethenzamide<br />
6.Bucetin<br />
1.Scopolamine Hydrobromide<br />
2.Atropine Sulfate<br />
1<br />
6<br />
1<br />
2<br />
2<br />
3<br />
4<br />
5<br />
0 6 12 18<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Methanol/50mM NH4H2PO4 - H3PO4 buffer(pH 2.5) 20/80<br />
Flow rate: 1.0 mL/min<br />
Det: 235 nm<br />
Oven: 40 ˚C<br />
0 4 8 12 16 20<br />
Min<br />
Column: Athena C18-WP 4.6 150mm, 5m (8.461572.0001)<br />
Eluent: Methanol/30mM NaH2PO4 buffer 15/85<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 40 ˚C
<strong>HPLC</strong> Application<br />
<br />
No.03259<br />
<br />
No.03258<br />
Carbamazepine<br />
1.Domiphen bromide<br />
1<br />
1<br />
9<br />
2 14 26 38 50<br />
Min<br />
Column: Athena CN 4.6 250mm, 5m (8.462533.0001)<br />
Eluent: Methanol/water/ Trifluoroacetic acid12/85/3<br />
Flow rate : 1.0 mL/min<br />
Det: 230 nm<br />
Oven: 25 ˚C<br />
0 2 4 6 8 10 12<br />
Min<br />
Column: Athena SCX 4.6 250mm, 5m (8.462523.0001)<br />
Eluent: Methanol/50mM Ammonium acetate80/20<br />
Flow rate: 0.7 mL/min<br />
Det: 274 nm<br />
Oven: 25 ˚C<br />
<br />
<br />
No.03252<br />
<br />
No.03261<br />
1.Acetylacetone<br />
2.1-Nitronaphthalene<br />
3.Naphthalene<br />
1 2<br />
1.Methanol<br />
2.Methotrexate<br />
3<br />
1<br />
2<br />
0 5 10 15<br />
Min<br />
20<br />
0 2 4 6 8 10<br />
Min<br />
Column: Athena C18 4.6 150mm, 5m (8.461571.0001)<br />
Eluent: 30mM Na3PO4 bufferH3PO4 - pH2.5<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 40 ˚C<br />
Column: Athena HILIC 4.6 150mm, 5m (8.461531.0001)<br />
Eluent: Acetonitrile /10mM Ammonium acetate90/10<br />
Flow rate: 1.0 mL/min<br />
Det: 306 nm<br />
Oven: 25 ˚C
No.03264<br />
<br />
No.03270<br />
1.Heparin Sodium<br />
1.Cefuroxime Sodium<br />
1<br />
1<br />
10<br />
<br />
0 12 24 36<br />
0 10 20 30<br />
Min<br />
Min<br />
Column:<br />
MRCGel X3010 7.8 300mm, 3m (8.7830AA.0001)<br />
Eluent: 0.2 mol/L Na2SO4 (H2SO4 to pH 3.0)<br />
Flow rate:<br />
Det:<br />
Oven:<br />
0.5 mL/min<br />
214 nm<br />
45 ˚C<br />
Column: Athena C18 4.6 250mm, 5m (8.462571.0001)<br />
Eluent: CH3COONa-CH3COOH bufferpH3.4/ Acetonitrile =(10/1)<br />
Flow rate: 1.5 mL/min<br />
Det: 254 nm<br />
Oven: 25 ˚C<br />
<br />
No.03265<br />
<br />
No.03269<br />
1.Rifampicin quinine<br />
2.Rifampicin<br />
3.N-O- Rifampicin<br />
1.Cefepime Hydrochloride<br />
2<br />
1<br />
1<br />
3<br />
4<br />
0 5 10 15 20 25<br />
Min<br />
0 2 4 6<br />
Min<br />
8<br />
Column: Athena C8 4.6 150mm, 5m (8.461575.0001)<br />
Eluent: Methanol Acetonitrile /0.075mMK2HPO4/1M Citric acid<br />
(30/30/36/4<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 25 ˚C<br />
Column: Athena C18 4.6 250mm, 5m (8.462571.0001)<br />
Eluent: Acetonitrile /Na2HPO4 buffer(pH 3.0)11/89<br />
Flow rate: 1.0 mL/min<br />
Det: 270 nm<br />
Oven: 25 ˚C
<strong>HPLC</strong> Application<br />
Applicationbiology<br />
<br />
No.03256<br />
<br />
1.Cytosine<br />
2.Uracil<br />
3.Cytidine<br />
4.Uridine<br />
5.Thymine<br />
No.03239<br />
1.Uridine<br />
2.Cytidine<br />
3.Guanosine<br />
4.Inosine and impurities<br />
5.Adenosine<br />
4<br />
2<br />
1<br />
1<br />
2<br />
4 5<br />
3<br />
3<br />
5<br />
11<br />
0 2 4 6 8 10 12<br />
Min<br />
Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001)<br />
Eluent: 100%Water<br />
Flow rate: 1.0 mL/min<br />
Det: 254 nm<br />
Oven: 40 ˚C<br />
2 4 6 8 10 12 14<br />
Min<br />
Column: Athena SCX 4.6 150mm, 5m (8.461523.0001)<br />
Eluent: 50mM Na2HPO4 buffer(pH 2.5)<br />
Flow rate: 0.5 mL/min<br />
Det: 280 nm<br />
Oven: 25 ˚C<br />
<br />
<br />
No.03254<br />
<br />
No.03250<br />
1.Cys-Cys(Acm)-Phe-Arg-Ser-Cys-Asy<br />
1<br />
0 10 20 30 40 50<br />
Min<br />
5 15 25 35<br />
Min<br />
Column: Athena C4 4.6 250mm, 5m (8.462579.0001)<br />
Eluent: A: 0.09%TFA; B: 0.085% TFA +80% Acetonitrile<br />
0min B 5%; 5min B 5%; 35min B 50%; 45min B 100%<br />
Flow rate: 1.0 mL/min<br />
Det: 214 nm<br />
Oven: 25 ˚C<br />
Column: Athena C18 4.6 150mm, 5m (8.461571.0001)<br />
Eluent:<br />
Flow rate:<br />
Det:<br />
Oven:<br />
A: 10mM TFA; B:10mM+60% Acetonitrile<br />
0min to 30min B from 0% to 100%<br />
1.0 mL/min<br />
214 nm<br />
25 ˚C