Applications/Columns HPLC - Mrclab.com

HPLC Application 

 

Food 

 

No.03276 

 

No.03271 

1.Citrus Red 2 

1.Bezoic Acid 

2.Sorbic acid 

1 

2 

1 

1 

0.0 

2.5 5.0 7.5 

Min 

0 5 10 

Min 

15 

 

Column : Athena C18-WP 4.6 150mm, 5m (8.461572.0001) 

Eluent: Acetonitrile/water(80/20) 

Flow Rate: 1.0 mL/min 

Det: 500 nm 

Temp: 35 ˚C 

Column: Athena C18-WP 4.6 250mm, 5m (8.462572.0001) 

Eluent: 20mM Ammonium acetate/Methanol (90/10) 


Det: 230 nm 

Temp: 25 ˚C 

 

No.03268 

 

No.03274 

1.New Red 

2.Tartrazine 

3.Amaranth 

4.Indigotine 

5.Ponceau 4RC 

6.Sunset Yellow 

7.Brilliant Blue FCF 

8.Erythrosin B disodium salt 

1.Sudan I 

2.Sudan II 

3.Sudan III 

4.Sudan IV 

1 

8 

1 

2 

3 

4 

5 

6 

2 

3 

4 

7 

0 

5 10 15 

Min 

0 5 10 

Min 


Eluent: A: MethanolB20mM Ammonium acetate (pH 4.0) 

0min B:80%5min B:65%12min B:2% 


Det: 254 nm 

Temp: 25 ˚C 


Eluent: Acetonitrile /Water (95/5) 


Det: 500 nm 

Temp: 35 ˚C

No.03267 

 

No.03231 

1.Nicotinamide 

1 

1.Glucose 

2.L-xylose 

3.Fructose 

4.Lyxose 

3 

4 

1 2 

2 

0 5 10 

Min 

0 4 8 12 16 20 

Min 


Column: 

MRCSep Ca-H 7.8 300mm, 5m (8.7830C3.0001) 

 

Eluent: Methanol / 2-Propanol /5mMSodium 1-heptanesulfonate 

7/2/91)Perchloric acid PH 2.1 


Det: 261 nm 

Eluent: 

Flow Rate: 

Det: 

Temp: 

Water 

0.6 mL/min 

192 nm 

85 ˚C 

Temp: 

25 ˚C 

 

No.03253 

 

No.03257 

1.Thiamin HCl 

2.Riboflavin 

7 

8 

9 

10 

1 

11 

12 

2 

13 

14 

15 

16 1718 

19 20 

21 22 23 24 25 26 

0 5 

10 15 

Min 

20 25 

5 15 25 35 

Min 

Column: Athena C18 4.6 150mm, 5m (8.461571.0001) 

Eluent: Acetonitrile /10mM Na3PO4 (pH 5.0)=15/85 


Det: 254 nm 

Temp: 40 ˚C 


Eluent: Methanol/Water2.5/97.5 


Det: RID 

Temp: 40 ˚C


 

No.03296 

Industrial 

1.-Tocopherol 

2.-Tocopherol 

3.-Tocopherol 

4.-Tocopherol 

 

No.03280 

1 

4 

1.BBP 

2.DBP 

3.DNHP 

4.DEHP 

5.DNOP 

6.DINP 

7.DIDP 

3 

2 

4 

1 

2 3 

5 

3 

6 

7 

0 10 20 30 

Min 

Column: Athena Silica 4.6 150mm, 5m (8.461576.0001) 

Eluent: N-hexan/ethanol 99/1 


Det: 280 nm 

Temp: 40 ˚C 

0 5 10 15 20 25 

Min 


Eluent: t(min) Acetonitrile Water Eluent (mL/min) 

0 90 10 1.0 

6.5 100 0 1.5 

7.5 100 0 1.5 

Det: 228 nm 

Temp: 25 ˚C 

 

 

No.03247 

1.Vitamin C 

2.Vitamin B13 

3.Nicotinic acid 

4.Nicotinamide 

5.Pyridoxal 

6.Pyridoxine 

7.Calcium pantothenate 

8.Thiamin 

Pesticide 

 

No.03260 

1 

1.Glyphosate 

1 

2 

3 4 

6 

5 

8 

7 

0 2 4 6 8 10 12 

Min 

0 2 4 6 8 

Min 


Eluent: Acetonitrile/20mM H3PO4+5mM 1-pentanesulfonate sodium 

buffer 8/92 


Det: 210 nm 

Temp: 40 ˚C 

Column: Athena SAX 4.6 150mm, 5m (8.461521.0001) 

Eluent: methanol/10mM KH2PO4(pH 2.0)(15/85) 


Det: 195 nm 

Temp: 25 ˚C

No.03277 

Application veterinary drugs 

1.Aldicarb-sulfoxide 

2.Aldicarb-sulfone 

3.Methomyl 

4.Carbofuran-3-hydroxy 

3 

5.Aldicarb 

6.Carbofuran 

7.Carbaryl 

7 

 

1.Norfloxacin 

2.Ciprofloxacin 

1 

No.03278 

1 

5 

2 

4 

6 

2 

4 

10 15 

20 

Min 

 


Eluent: t(min) Water% Methnal% Flow rate ( ml/min) 

0 85 15 0.5 

2 75 25 0.5 

8 75 25 0.5 

9 60 40 0.8 

10 55 45 0.8 

19 20 80 0.8 

25 20 80 0.8 

26 85 15 0.5 

Det: 

FLDex 330nmem 465nm 

post-column 

derivation: 

Oven: 

50mM NaOH and OPAflow rate 0.3ml/minhydrolyze 

Temp 100Cderivation : room temp 

42 ˚C 

0 5 10 

Min 


Eluent : 25mM –PO4 Buffer/ Acetonitrile (85/15) 

Flow rate: 1.0 mL/min 

Det: 254 nm 

Oven: 25 ˚C 

 

No.03248 

 

No.03251 

1.Thiabendazole 

2.o-Phenylphenol 

3.Biphenyl 

2 

3 

1.Sulfanilamide 

2.Sulfisomidine 

3.Sulfadiazine 

4.Sulfamethazine 

5.Sulfamonomethoxine 

1 

1 

2 

4 

3 

5 

0 2 4 6 8 10 12 

Min 

0 5 15 20 25 

Min 


Eluent: Acetonitrile /30mM NH4H2PO4 buffer65/35 


Det: 254 nm 

Oven: 40 ˚C 


Eluent: Acetonitrile /10mM H3PO4 buffer15/85 


Det: 254 nm 

Oven: 40 ˚C


 

No.03273 

 

No.03272 

1.Sulfadiazine 

2.Sulfamethazine 

3.Sulfamonomethoxine 

4.Sulfamethoxazole 

5.Sulfachinoxalin 

1.Oxytetracycline 

2.Tetracycline 

3.Chlortetracycline 

1 

2 

1 

3 

2 

4 

3 

5 

0.0 2.5 5.0 7.5 10.0 

Min 

5 

0 5 10 15 

Min 


Eluent: Acetonitrile /water/ acetic acid (25/75/0.3) 


Det: 270 nm 

Oven: 25 ˚C 

Application environment 

 

1.o-nitroaniline 

2.m-nitroaniline 

3.p-nitroaniline 

2 

20 

No.03297 


Eluent: Acetonitrile /methnal/10mM Oxalic acid (15/15/70) 


Det: 355 nm 

Oven: 25 ˚C 

 

1.Oxamyl 

2.Methomyl 

3.Aldicarb 

4.Simazine 

5.Monuron 

6.Cyanazine 

7.Metribuzin 

8.Carbofuran 

56 

1 2 4 

7 

9.Atrazine 

10.Carbaryl 

10.Fluometuron 

11.Diuron 

12.Propham 

13.Propachlor 

14.Linuron 

9 10 11 12 13 14 

No.03275 

 

1 

3 

3 

8 

0 8 16 

24 

0 5 10 15 20 25 

Min 

Column: Athena Silica 4.6 150mm, 5m (8.461576.0001) 

Eluent: N-HEXAN / TRICHLORMETHAN40/60 


Det: 254 nm 

Oven: 25 ˚C 

Min 


Eluent: A: Water/ Acetonitrile (90/10)BAcetonitrile 

0min B:20%5min B:20%30min B:70% 


Det: 220 nm 

Oven: 25 ˚C

1.Naphthalene 

2.Acenaphthylene 

3.Fluorene 

4.Acenaphthene 

5.Phenanthrene 

6.Anthracene 

7.Fluoranthene 

8.Pyrene 

9.Chrysene 

No.03266 

10.Benzo(a)anthracene 

11.Benzo(b)fluoranthene 

12.Benzo(k)fluoranthene 

13.Benzo(a)pyrene 

14.Dibenzo(a,h)anthracene 

15.Benzo(g,h,i)perylene 

16.Indeno(1,2,3-cd)pyrene 

Application Pharmaceuticals 

 

1.Piroxicam 

2.Sulindac 

3.Ketoprofen 

 

4.Naproxen 

5.Flurbiprofen 

6.Ibuprofen 

No.03237 

6 

 

 

6 

 

0 

2 

1 

3 

4 

5 


Eluent: A: AcetonitrileBwater 

0min B:35%27 min B:35%41min B:0% 


Det: 254 nm 

Oven: 25 ˚C 

7 

8 

9 

10 

12 

11 13 

15 30 45 

Min 

14 

15,16 

 

 

 

0 5 10 15 

Min 


Eluent: Acetonitrile /1% acetic acid buffer65/35 


Det: 260 nm 

Oven: 40 ˚C 

 

No.03238 

 

1.Bishphenol A 

No.03281 

1.Protriptyline 

2.Desipramine 

3.Nortriptyline 

4.Doxepin 

5.Imipramine 

6.Amitriptyline 

7.Trimipramine 

1 

2 

3 

1 

4 

6 

5 

7 

0 1 2 3 4 5 6 7 

Min 


Eluent: AAcetonitrileBWater 

0min B:40%7min B:5% 


Det: 216 nm 

Oven: 35 ˚C 

0 

15 30 

Min 


Eluent: Methnal/20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 70/30 


Det: 240 nm 

Oven: 30 ˚C


 

No.03249 

 

No.03243 

1. Estriol 

2.-Estradiol 

3.Estrone 

4.Progesterone 

1.Cinchonine 

2.Cinchonidine 

3.Quinidine 

4.Quinine 

1 

2 

3 

4 

1 2 

3 4 

7 

0 4 8 12 16 20 

Min 


Eluent: 

Flow rate: 

Det: 

Oven: 

Acetonitrile /Water60/40 

1.0 mL/min 

30 nm 

35 ˚C 

0 6 12 18 

Min 


Eluent: 

Flow rate: 

Det: 

Oven: 

Methanol/20mM KH2PO4 buffer35/65 

1.0 mL/min 

235 nm 

40 ˚C 

 

No.03240 

 

No.03246 

1.Pheniramine 

2.Doxylamine 

3.Chlorpheniramine 

4.Diphenhydramine 

1.Primidone 

2.Phenytoin 

3.Carbamazepine 

3 

2 

4 

1 

2 

1 

3 

0 6 12 18 24 30 

Min 

0 6 

Min 

12 18 


Eluent: Methanol/20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 60/40 


Det: 254 nm 

Oven: 40 ˚C 


Eluent: Acetonitrile /20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 70/30 


Det: 220 nm 

Oven: 30 ˚C

No.03241 No.03242 

1.Atenolol 

2.Nadolol 

3.Metoprolol 

4.Propranolol 

5.Alprenolol 

1.Uracil 

2.Procainamide 

3.N-Acetylprocainamide 

1 

3 

2 

3 

1 

2 

4 

5 

8 

 

0 5 10 

Min 


Eluent: Methanol/20mM KH2PO4 - K2HPO4 buffer(pH 7.0)60/40 


Det: 220 nm 

Oven: 40 ˚C 

0 2 4 6 8 10 

Min 


Eluent: Methanol/20mM KH2PO4 - K2HPO4 buffer(pH 7.0) 40/60 


Det: 254 nm 

Oven: 40 ˚C 

 

No.03245 

 

No.03244 

1.Acetaminophen 

2.Caffeine 

3.Chlorpheniramine Maleate 

4.Aspirine 

5.Ethenzamide 

6.Bucetin 

1.Scopolamine Hydrobromide 

2.Atropine Sulfate 

1 

6 

1 

2 

2 

3 

4 

5 

0 6 12 18 

Min 


Eluent: Methanol/50mM NH4H2PO4 - H3PO4 buffer(pH 2.5) 20/80 


Det: 235 nm 

Oven: 40 ˚C 

0 4 8 12 16 20 

Min 


Eluent: Methanol/30mM NaH2PO4 buffer 15/85 


Det: 254 nm 

Oven: 40 ˚C


 

No.03259 

 

No.03258 

Carbamazepine 

1.Domiphen bromide 

1 

1 

9 

2 14 26 38 50 

Min 

Column: Athena CN 4.6 250mm, 5m (8.462533.0001) 

Eluent: Methanol/water/ Trifluoroacetic acid12/85/3 

Flow rate : 1.0 mL/min 

Det: 230 nm 

Oven: 25 ˚C 

0 2 4 6 8 10 12 

Min 

Column: Athena SCX 4.6 250mm, 5m (8.462523.0001) 

Eluent: Methanol/50mM Ammonium acetate80/20 


Det: 274 nm 

Oven: 25 ˚C 

 

 

No.03252 

 

No.03261 

1.Acetylacetone 

2.1-Nitronaphthalene 

3.Naphthalene 

1 2 

1.Methanol 

2.Methotrexate 

3 

1 

2 

0 5 10 15 

Min 

20 

0 2 4 6 8 10 

Min 


Eluent: 30mM Na3PO4 bufferH3PO4 - pH2.5 


Det: 254 nm 

Oven: 40 ˚C 

Column: Athena HILIC 4.6 150mm, 5m (8.461531.0001) 

Eluent: Acetonitrile /10mM Ammonium acetate90/10 


Det: 306 nm 

Oven: 25 ˚C

No.03264 

 

No.03270 

1.Heparin Sodium 

1.Cefuroxime Sodium 

1 

1 

10 

 

0 12 24 36 

0 10 20 30 

Min 

Min 

Column: 

MRCGel X3010 7.8 300mm, 3m (8.7830AA.0001) 

Eluent: 0.2 mol/L Na2SO4 (H2SO4 to pH 3.0) 

Flow rate: 

Det: 

Oven: 

0.5 mL/min 

214 nm 

45 ˚C 


Eluent: CH3COONa-CH3COOH bufferpH3.4/ Acetonitrile =(10/1) 


Det: 254 nm 

Oven: 25 ˚C 

 

No.03265 

 

No.03269 

1.Rifampicin quinine 

2.Rifampicin 

3.N-O- Rifampicin 

1.Cefepime Hydrochloride 

2 

1 

1 

3 

4 

0 5 10 15 20 25 

Min 

0 2 4 6 

Min 

8 


Eluent: Methanol Acetonitrile /0.075mMK2HPO4/1M Citric acid 

(30/30/36/4 


Det: 254 nm 

Oven: 25 ˚C 


Eluent: Acetonitrile /Na2HPO4 buffer(pH 3.0)11/89 


Det: 270 nm 

Oven: 25 ˚C


Applicationbiology 

 

No.03256 

 

1.Cytosine 

2.Uracil 

3.Cytidine 

4.Uridine 

5.Thymine 

No.03239 

1.Uridine 

2.Cytidine 

3.Guanosine 

4.Inosine and impurities 

5.Adenosine 

4 

2 

1 

1 

2 

4 5 

3 

3 

5 

11 

0 2 4 6 8 10 12 

Min 


Eluent: 100%Water 


Det: 254 nm 

Oven: 40 ˚C 

2 4 6 8 10 12 14 

Min 

Column: Athena SCX 4.6 150mm, 5m (8.461523.0001) 

Eluent: 50mM Na2HPO4 buffer(pH 2.5) 


Det: 280 nm 

Oven: 25 ˚C 

 

 

No.03254 

 

No.03250 

1.Cys-Cys(Acm)-Phe-Arg-Ser-Cys-Asy 

1 

0 10 20 30 40 50 

Min 

5 15 25 35 

Min 


Eluent: A: 0.09%TFA; B: 0.085% TFA +80% Acetonitrile 

0min B 5%; 5min B 5%; 35min B 50%; 45min B 100% 


Det: 214 nm 

Oven: 25 ˚C 


Eluent: 

Flow rate: 

Det: 

Oven: 

A: 10mM TFA; B:10mM+60% Acetonitrile 

0min to 30min B from 0% to 100% 

1.0 mL/min 

214 nm 

25 ˚C

Applications/Columns HPLC - Mrclab.com

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