Identification of free-living heterotrophic flagellates by fluorescence ...

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FISH in flagellates

• sensitivity:

! fluorescent dyes

! HRP-labeled probes with TSA

• autofluorescence of chlorophyll

! UV-excitable fluorochrome Alexa-350

BioBaz Campagnes Couv - Station Biologique de Roscoff

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THESE Anne POSTEC DiversitÃƒÂ© de populations microbiennes ...

Geochemical energy sources for microbial primary production in the ...

FISH in flagellates • sensitivity: ! fluorescent dyes ! HRP-labeled probes with TSA • autofluorescence of chlorophyll ! UV-excitable fluorochrome Alexa-350

Hybridization with HRP-labeled probes: HRP-probes + flagellate cell ribosomes Incubation with Alexa350-labeled tyramides:

Page 1 and 2: Identification of free-living heter
Page 3 and 4: Common methods • light microscopy
Page 5: 0.1 Different trophic modes in clos
Page 9 and 10: Development of group specific probe
Page 11 and 12: Mixed culture of Pteridomonas danic
Page 13 and 14: Conclusion FISH + HRP Phylogeny Sen
Page 15: Conclusion FISH + HRP + UV-dye Phyl

Identification of free-living heterotrophic flagellates by fluorescence ...

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