Novocastratm Ready-to-Use Mouse Monoclonal Antibody CD20

NovocastraTM Ready-to-Use Mouse Monoclonal Antibody
CD20
Product Code: RTU-CD20-L26
Intended Use
For in vitro diagnostic use.
RTU-CD20-L26 is intended for the qualitative identifi cation by light microscopy of CD20 molecules in paraffi n sections. The clinical
interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be
evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualifi ed pathologist.
Principle of Procedure
Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specifi c antibody
to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate
with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The
specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential
diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
Clone
L26
Immunogen
Human tonsil B cells.
Specifi city
An intracytoplasmic epitope localised on the human CD20 molecule. Reacts predominantly with a 33 kD polypeptide, but also with a
minor component of 30 kD.
Reagent Composition
RTU-CD20-L26 is a ready to use liquid tissue culture supernatant, presented in 5% horse serum in PBS containing 12 mM sodium azide
as a preservative.
Ig Class
IgG2a
Total Protein
Total Protein Concentration
Range 1.0–8.0 g/L. Refer to vial label for lot specifi c total protein concentration.
Antibody Concentration
Greater than or equal to 2.1 mg/L as determined by ELISA. Refer to vial label for batch specifi c Ig concentration.
Recommendations On Use
Immunohistochemistry (see D. Methodology) on paraffi n sections. Incubate tissue section with primary reagent for 15 minutes at 25 o C.
High temperature antigen retrieval using 0.01 M citrate retrieval solution (pH 6.0) is recommended. This antibody is pre-titred for use and
does not require further dilution when used with the secondary detection system, RE7100-K.
Storage and Stability
Store at 2–8 o C. Do not freeze. Return to 2–8 o C immediately after use. Do not use after expiration date indicated on the vial label.
Storage conditions other than those specifi ed above must be verifi ed by the user.
Specimen Preparation
The recommended fi xative is 10% neutral-buffered formalin for paraffi n-embedded tissue sections.
Warnings and Precautions
This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when
handling it.
The molarity of sodium azide in this reagent is 12 mM. A Material Safety Data Sheet (MSDS) is available upon request for sodium azide.
Consult federal, state or local regulations for disposal of any potentially toxic components.
Specimens, before and after fi xation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions. 1 Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
Minimize microbial contamination of reagents or an increase in non-specifi c staining may occur.
Incubation times or temperatures, other than those specifi ed, may give erroneous results. Any such changes must be validated by the
user.
Quality Control
Differences in tissue processing and technical procedures in the user’s laboratory may produce signifi cant variability in results,
necessitating regular performance of in-house controls in addition to the following procedures.
Controls should be fresh autopsy/biopsy/surgical specimens, formalin-fi xed, processed and paraffi n wax-embedded as soon as possible
in the same manner as the patient sample(s).
RTU-CD20-L26
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