Mycotrim® RS - Irvine Scientific

M. pneumoniae colony appearance:
M. pneumoniae colonies are 60 to 200 microns in diameter,
about 1/10 as large as a small bacterial colony, 1 to 5 times
larger than a single buccal epithelial cell, and 10 to 15 times
larger than a single white blood cell. Mycoplasma colonies
have a characteristic “fried egg” or “sand patty” (grainy)
appearance. Epithelial cells are clearly distinguished from
mycoplasma colonies by their sharp, angular margins and
clear cytoplasm (often folded over upon itself).
5. Reinoculation
Do not open the Mycotrim RS flask.
If no color change occurs after 72 hours, reinoculate the agar
by tilting the flask so that the broth contacts 1/3 to 1/2 of
the agar surface, as shown in Figure 4. After reinoculation,
incubate the flask at 34° C to 37° C with the agar side up.
Continue to examine the flask daily for a color change. When
the color change is observed, look for typical microscopic
colonies (see B. Colony Appearance, above).
English 8/10
Figure 4